Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier

Y. Date, K. Isaka, T. Sumino, S. Tsuneda, Y. Inamori

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

Anaerobic ammonium oxidation (anammox) is a recently discovered microbial pathway in the biological nitrogen cycle and a new cost-effective way to remove ammonium from wastewater. We have so far developed new immobilization technique that anammox bacteria entrapped in polyethylene glycol (PEG) gel carrier. However, fate and behavior of anammox bacteria in a gel carrier is not well understood. In the present study, we focused on the population changes of anammox bacteria in a gel carrier. Three specific primer sets were designed for real-time PCR. For quantification of anammox bacteria in a gel carrier, real-time PCR was performed. The anammox bacteria related to HPT-WU-N03 clone were increased the rate in anammox population, and found to be a major population of anammox bacteria in a gel carrier. Furthermore, from the results of nitrogen removal performance and quantification of anammox bacteria, the correlation coefficient between copy numbers of anammox bacteria and nitrogen conversion rate was calculated as 0.947 in total anammox population. This is the first report that population changes of anammox bacteria immobilized in a gel carrier were evaluated.

Original languageEnglish
Title of host publicationWater Science and Technology
Pages1121-1128
Number of pages8
Edition5
DOIs
Publication statusPublished - 2008 Nov 10

Publication series

NameWater Science and Technology
Number5
Volume58
ISSN (Print)0273-1223

Keywords

  • Anaerobic ammonium oxidation (anammox)
  • Community structure
  • FISH
  • Immobilization
  • Planctomycetes
  • Real-time PCR

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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  • Cite this

    Date, Y., Isaka, K., Sumino, T., Tsuneda, S., & Inamori, Y. (2008). Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. In Water Science and Technology (5 ed., pp. 1121-1128). (Water Science and Technology; Vol. 58, No. 5). https://doi.org/10.2166/wst.2008.466