Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier

Y. Date, K. Isaka, T. Sumino, Satoshi Tsuneda, Y. Inamori

    Research output: Chapter in Book/Report/Conference proceedingChapter

    10 Citations (Scopus)

    Abstract

    Anaerobic ammonium oxidation (anammox) is a recently discovered microbial pathway in the biological nitrogen cycle and a new cost-effective way to remove ammonium from wastewater. We have so far developed new immobilization technique that anammox bacteria entrapped in polyethylene glycol (PEG) gel carrier. However, fate and behavior of anammox bacteria in a gel carrier is not well understood. In the present study, we focused on the population changes of anammox bacteria in a gel carrier. Three specific primer sets were designed for real-time PCR. For quantification of anammox bacteria in a gel carrier, real-time PCR was performed. The anammox bacteria related to HPT-WU-N03 clone were increased the rate in anammox population, and found to be a major population of anammox bacteria in a gel carrier. Furthermore, from the results of nitrogen removal performance and quantification of anammox bacteria, the correlation coefficient between copy numbers of anammox bacteria and nitrogen conversion rate was calculated as 0.947 in total anammox population. This is the first report that population changes of anammox bacteria immobilized in a gel carrier were evaluated.

    Original languageEnglish
    Title of host publicationWater Science and Technology
    Pages1121-1128
    Number of pages8
    Volume58
    Edition5
    DOIs
    Publication statusPublished - 2008

    Publication series

    NameWater Science and Technology
    Number5
    Volume58
    ISSN (Print)02731223

    Fingerprint

    Polyethylene glycols
    microbial community
    Bacteria
    ammonium
    Gels
    gel
    oxidation
    Oxidation
    bacterium
    Nitrogen
    Nitrogen removal
    nitrogen cycle
    nitrogen
    immobilization
    Wastewater
    clone
    wastewater

    Keywords

    • Anaerobic ammonium oxidation (anammox)
    • Community structure
    • FISH
    • Immobilization
    • Planctomycetes
    • Real-time PCR

    ASJC Scopus subject areas

    • Environmental Engineering
    • Water Science and Technology

    Cite this

    Date, Y., Isaka, K., Sumino, T., Tsuneda, S., & Inamori, Y. (2008). Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. In Water Science and Technology (5 ed., Vol. 58, pp. 1121-1128). (Water Science and Technology; Vol. 58, No. 5). https://doi.org/10.2166/wst.2008.466

    Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. / Date, Y.; Isaka, K.; Sumino, T.; Tsuneda, Satoshi; Inamori, Y.

    Water Science and Technology. Vol. 58 5. ed. 2008. p. 1121-1128 (Water Science and Technology; Vol. 58, No. 5).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Date, Y, Isaka, K, Sumino, T, Tsuneda, S & Inamori, Y 2008, Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. in Water Science and Technology. 5 edn, vol. 58, Water Science and Technology, no. 5, vol. 58, pp. 1121-1128. https://doi.org/10.2166/wst.2008.466
    Date Y, Isaka K, Sumino T, Tsuneda S, Inamori Y. Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. In Water Science and Technology. 5 ed. Vol. 58. 2008. p. 1121-1128. (Water Science and Technology; 5). https://doi.org/10.2166/wst.2008.466
    Date, Y. ; Isaka, K. ; Sumino, T. ; Tsuneda, Satoshi ; Inamori, Y. / Microbial community of anammox bacteria immobilized in polyethylene glycol gel carrier. Water Science and Technology. Vol. 58 5. ed. 2008. pp. 1121-1128 (Water Science and Technology; 5).
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