Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor

Yasuhiro Date, Kazuichi Isaka, Hajime Ikuta, Tatsuo Sumino, Naoya Kaneko, Sachiko Yoshie, Satoshi Tsuneda, Yuhei Inamori

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Enrichment of anammox bacteria from three types of seed sludge, sewage, digester, and nitrification sludges, was conducted using a nonwoven fabric carrier for immobilizing the anammox bacteria, and the microbial diversity of the enriched anammox culture was investigated. About four months later, simultaneous removals of ammonium and nitrite, and production of a small amount of nitrate, which is unique to the anammox reaction, were observed in all 3 sludge reactors. Results of 16S rRNA gene analysis indicated that anammox bacteria were cultivated and diversified in each sludge type. Moreover, the microbial diversity of anammox bacteria was higher in the enriched culture from sewage sludge compared to the other two types of seed sludge. Bacillus sp. coexisted in the anammox culture cultivated from sewage sludge. These results suggest that differences in the anammox community in the enriched culture were caused by differences in the type of seed sludge.

    Original languageEnglish
    Pages (from-to)281-286
    Number of pages6
    JournalJournal of Bioscience and Bioengineering
    Volume107
    Issue number3
    DOIs
    Publication statusPublished - 2009 Mar

    Fingerprint

    Sewage sludge
    Sewage
    Seed
    Seeds
    Bacteria
    Nonwoven fabrics
    Nitrification
    Bacilli
    Nitrites
    Ammonium Compounds
    Nitrates
    Genes
    rRNA Genes
    Bacillus

    Keywords

    • Anaerobic ammonium oxidation (anammox)
    • Microbial diversity
    • Nitrogen removal
    • Nonwoven fabric carrier
    • Phylogenetic analysis
    • Reproducible enrichment

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor. / Date, Yasuhiro; Isaka, Kazuichi; Ikuta, Hajime; Sumino, Tatsuo; Kaneko, Naoya; Yoshie, Sachiko; Tsuneda, Satoshi; Inamori, Yuhei.

    In: Journal of Bioscience and Bioengineering, Vol. 107, No. 3, 03.2009, p. 281-286.

    Research output: Contribution to journalArticle

    Date, Yasuhiro ; Isaka, Kazuichi ; Ikuta, Hajime ; Sumino, Tatsuo ; Kaneko, Naoya ; Yoshie, Sachiko ; Tsuneda, Satoshi ; Inamori, Yuhei. / Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor. In: Journal of Bioscience and Bioengineering. 2009 ; Vol. 107, No. 3. pp. 281-286.
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    abstract = "Enrichment of anammox bacteria from three types of seed sludge, sewage, digester, and nitrification sludges, was conducted using a nonwoven fabric carrier for immobilizing the anammox bacteria, and the microbial diversity of the enriched anammox culture was investigated. About four months later, simultaneous removals of ammonium and nitrite, and production of a small amount of nitrate, which is unique to the anammox reaction, were observed in all 3 sludge reactors. Results of 16S rRNA gene analysis indicated that anammox bacteria were cultivated and diversified in each sludge type. Moreover, the microbial diversity of anammox bacteria was higher in the enriched culture from sewage sludge compared to the other two types of seed sludge. Bacillus sp. coexisted in the anammox culture cultivated from sewage sludge. These results suggest that differences in the anammox community in the enriched culture were caused by differences in the type of seed sludge.",
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    AU - Kaneko, Naoya

    AU - Yoshie, Sachiko

    AU - Tsuneda, Satoshi

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