TY - JOUR
T1 - Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization
AU - Aoi, Yoshiteru
AU - Miyoshi, Tomoko
AU - Okamoto, Toshiyuki
AU - Tsuneda, Satoshi
AU - Hirata, Akira
AU - Kitayama, Atsushi
AU - Nagamune, Teruyuki
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.
AB - The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.
KW - Ammonia-oxidizing bacteria
KW - Fluorescence in situ hybridization
KW - Microbial ecology
KW - Nitrification
UR - http://www.scopus.com/inward/record.url?scp=0034281838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034281838&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(00)80075-4
DO - 10.1016/S1389-1723(00)80075-4
M3 - Article
C2 - 16232850
AN - SCOPUS:0034281838
SN - 1389-1723
VL - 90
SP - 234
EP - 240
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 3
ER -