Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization

Yoshiteru Aoi, Tomoko Miyoshi, Toshiyuki Okamoto, Satoshi Tsuneda, Akira Hirata, Atsushi Kitayama, Teruyuki Nagamune

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.

    Original languageEnglish
    Pages (from-to)234-240
    Number of pages7
    JournalJournal of Bioscience and Bioengineering
    Volume90
    Issue number3
    DOIs
    Publication statusPublished - 2000 Sep

    Fingerprint

    Ecology
    Waste Water
    Fluorescence In Situ Hybridization
    Wastewater treatment
    Bacteria
    Fluorescence
    Ammonia
    Biofilms
    Wastewater
    Oligonucleotide Probes
    Oligonucleotides
    Substrates
    Chemical analysis
    Spatial distribution
    Dynamic response

    Keywords

    • Ammonia-oxidizing bacteria
    • Fluorescence in situ hybridization
    • Microbial ecology
    • Nitrification

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Cite this

    Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization. / Aoi, Yoshiteru; Miyoshi, Tomoko; Okamoto, Toshiyuki; Tsuneda, Satoshi; Hirata, Akira; Kitayama, Atsushi; Nagamune, Teruyuki.

    In: Journal of Bioscience and Bioengineering, Vol. 90, No. 3, 09.2000, p. 234-240.

    Research output: Contribution to journalArticle

    Aoi, Yoshiteru ; Miyoshi, Tomoko ; Okamoto, Toshiyuki ; Tsuneda, Satoshi ; Hirata, Akira ; Kitayama, Atsushi ; Nagamune, Teruyuki. / Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization. In: Journal of Bioscience and Bioengineering. 2000 ; Vol. 90, No. 3. pp. 234-240.
    @article{24273d80f1724788ac6da78cbc0ec54a,
    title = "Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization",
    abstract = "The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.",
    keywords = "Ammonia-oxidizing bacteria, Fluorescence in situ hybridization, Microbial ecology, Nitrification",
    author = "Yoshiteru Aoi and Tomoko Miyoshi and Toshiyuki Okamoto and Satoshi Tsuneda and Akira Hirata and Atsushi Kitayama and Teruyuki Nagamune",
    year = "2000",
    month = "9",
    doi = "10.1016/S1389-1723(00)80075-4",
    language = "English",
    volume = "90",
    pages = "234--240",
    journal = "Journal of Bioscience and Bioengineering",
    issn = "1389-1723",
    publisher = "Elsevier",
    number = "3",

    }

    TY - JOUR

    T1 - Microbial ecology of nitrifying bacteria in wastewater treatment process examined by fluorescence in situ hybridization

    AU - Aoi, Yoshiteru

    AU - Miyoshi, Tomoko

    AU - Okamoto, Toshiyuki

    AU - Tsuneda, Satoshi

    AU - Hirata, Akira

    AU - Kitayama, Atsushi

    AU - Nagamune, Teruyuki

    PY - 2000/9

    Y1 - 2000/9

    N2 - The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.

    AB - The microbial ecology of nitrifying bacteria in various types of wastewater treatment processes and the dynamic response of the microbial ecology in biofilms were investigated using fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. Nitrifying bacteria were found to exhibit various organizational forms under different conditions of substrate composition and concentration. Ammonia-oxidizing bacteria were dominant in ammonia-rich inorganic wastewater, while heterotrophic bacteria and ammonia-oxidizing bacteria were localized at different positions in the biofilm in organic wastewater. The dynamics of the microbial ecology in the biofilm with regard to the spatial distribution of ammonia-oxidizing bacteria and heterotrophic bacteria caused by a gradual change in substrate composition was successfully monitored by FISH analysis.

    KW - Ammonia-oxidizing bacteria

    KW - Fluorescence in situ hybridization

    KW - Microbial ecology

    KW - Nitrification

    UR - http://www.scopus.com/inward/record.url?scp=0034281838&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0034281838&partnerID=8YFLogxK

    U2 - 10.1016/S1389-1723(00)80075-4

    DO - 10.1016/S1389-1723(00)80075-4

    M3 - Article

    VL - 90

    SP - 234

    EP - 240

    JO - Journal of Bioscience and Bioengineering

    JF - Journal of Bioscience and Bioengineering

    SN - 1389-1723

    IS - 3

    ER -