Abstract
Microbial population dynamics were investigated during the formation of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor fed ammonia as a sole energy source. Analyses of clone libraries of 16S rRNA gene and the ammonia monooxygenase subunit A gene (amoA) revealed that although the clones obtained from the seed sludge were widely distributed among the ammonia-oxidizing bacteria (AOB) isolates, the community structure of AOB shifted towards the Nitrosomonas mobilis lineage as granulation proceeded. Quantitative fluorescence in situ hybridization showed that changes in the bacterial population occurred concomitantly with changes in nitrification performance and the size of granules. AOB associated with the N. mobilis lineage were predominant in the early stages as nitrifying granules formed (average diameter, 126 μm). In mature granules (average diameter, 270 μm), at least three types of AOB, N. mobilis, Nitrosomonas oligotropha, and Nitrosomonas europaea, formed different niches and coexisted. Nitrite-oxidizing bacteria (NOB) affiliated with Nitrospira spp. were detected in the start-up period, but were replaced by NOB affiliated with Nitrobacter spp. after granules formed.
| Original language | English |
|---|---|
| Pages (from-to) | 164-170 |
| Number of pages | 7 |
| Journal | Microbes and Environments |
| Volume | 25 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Community structure
- Nitrifying bacteria
- Population dynamics
ASJC Scopus subject areas
- Plant Science
- Soil Science
- Ecology, Evolution, Behavior and Systematics
Cite this
Microbial population dynamics and community structure during the formation of nitrifying granules to treat ammonia-rich inorganic wastewater. / Matsumoto, Shinya; Ishikawa, Daisuke; Saeki, Goro; Aoi, Yoshiteru; Tsuneda, Satoshi.
In: Microbes and Environments, Vol. 25, No. 3, 2010, p. 164-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microbial population dynamics and community structure during the formation of nitrifying granules to treat ammonia-rich inorganic wastewater
AU - Matsumoto, Shinya
AU - Ishikawa, Daisuke
AU - Saeki, Goro
AU - Aoi, Yoshiteru
AU - Tsuneda, Satoshi
PY - 2010
Y1 - 2010
N2 - Microbial population dynamics were investigated during the formation of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor fed ammonia as a sole energy source. Analyses of clone libraries of 16S rRNA gene and the ammonia monooxygenase subunit A gene (amoA) revealed that although the clones obtained from the seed sludge were widely distributed among the ammonia-oxidizing bacteria (AOB) isolates, the community structure of AOB shifted towards the Nitrosomonas mobilis lineage as granulation proceeded. Quantitative fluorescence in situ hybridization showed that changes in the bacterial population occurred concomitantly with changes in nitrification performance and the size of granules. AOB associated with the N. mobilis lineage were predominant in the early stages as nitrifying granules formed (average diameter, 126 μm). In mature granules (average diameter, 270 μm), at least three types of AOB, N. mobilis, Nitrosomonas oligotropha, and Nitrosomonas europaea, formed different niches and coexisted. Nitrite-oxidizing bacteria (NOB) affiliated with Nitrospira spp. were detected in the start-up period, but were replaced by NOB affiliated with Nitrobacter spp. after granules formed.
AB - Microbial population dynamics were investigated during the formation of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor fed ammonia as a sole energy source. Analyses of clone libraries of 16S rRNA gene and the ammonia monooxygenase subunit A gene (amoA) revealed that although the clones obtained from the seed sludge were widely distributed among the ammonia-oxidizing bacteria (AOB) isolates, the community structure of AOB shifted towards the Nitrosomonas mobilis lineage as granulation proceeded. Quantitative fluorescence in situ hybridization showed that changes in the bacterial population occurred concomitantly with changes in nitrification performance and the size of granules. AOB associated with the N. mobilis lineage were predominant in the early stages as nitrifying granules formed (average diameter, 126 μm). In mature granules (average diameter, 270 μm), at least three types of AOB, N. mobilis, Nitrosomonas oligotropha, and Nitrosomonas europaea, formed different niches and coexisted. Nitrite-oxidizing bacteria (NOB) affiliated with Nitrospira spp. were detected in the start-up period, but were replaced by NOB affiliated with Nitrobacter spp. after granules formed.
KW - Community structure
KW - Nitrifying bacteria
KW - Population dynamics
UR - http://www.scopus.com/inward/record.url?scp=77957093433&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957093433&partnerID=8YFLogxK
U2 - 10.1264/jsme2.ME10107
DO - 10.1264/jsme2.ME10107
M3 - Article
C2 - 21576869
AN - SCOPUS:77957093433
VL - 25
SP - 164
EP - 170
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 3
ER -