Abstract
A microbioassay of leucine is achieved by the use of Leuconostoc mesenteroides and a lactate sensor based on an oxygen electrode, which is coated with immobilized lactate oxidase. The response time of the lactate sensor observed by an injection method was about 1 min. Linear relationship was obtained between the current decrease and lactate concentration in the range from. When the microbioassay media containing leucine were incubated for 6 h with L. mesenteroides and introduced into the lactate sensor system, a relationship between the current decrease and the leucine concentration was linear over the range of The current decrease was reproducible within 1% when the media containing of leucine was used. The electrochemical system was applied to the analysis of leucine in human sera. The results obtained by the present method were in good agreement with those obtained by an amino acid autoanalyzer (6 experiments, leucine concentrations in human sera; correlation coefficient; 0,98).
| Original language | English |
|---|---|
| Pages (from-to) | 1537-1541 |
| Number of pages | 5 |
| Journal | Nippon Kagaku Kaishi |
| Volume | 1980 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Microbioassay of Amino Acid with a Lactate Sensor. / Matsunaga, Tadashi; Karube, Isao; Teraoka, Nobuaki; Suzuki, Shuichi.
In: Nippon Kagaku Kaishi, Vol. 1980, No. 10, 1980, p. 1537-1541.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microbioassay of Amino Acid with a Lactate Sensor
AU - Matsunaga, Tadashi
AU - Karube, Isao
AU - Teraoka, Nobuaki
AU - Suzuki, Shuichi
PY - 1980
Y1 - 1980
N2 - A microbioassay of leucine is achieved by the use of Leuconostoc mesenteroides and a lactate sensor based on an oxygen electrode, which is coated with immobilized lactate oxidase. The response time of the lactate sensor observed by an injection method was about 1 min. Linear relationship was obtained between the current decrease and lactate concentration in the range from. When the microbioassay media containing leucine were incubated for 6 h with L. mesenteroides and introduced into the lactate sensor system, a relationship between the current decrease and the leucine concentration was linear over the range of The current decrease was reproducible within 1% when the media containing of leucine was used. The electrochemical system was applied to the analysis of leucine in human sera. The results obtained by the present method were in good agreement with those obtained by an amino acid autoanalyzer (6 experiments, leucine concentrations in human sera; correlation coefficient; 0,98).
AB - A microbioassay of leucine is achieved by the use of Leuconostoc mesenteroides and a lactate sensor based on an oxygen electrode, which is coated with immobilized lactate oxidase. The response time of the lactate sensor observed by an injection method was about 1 min. Linear relationship was obtained between the current decrease and lactate concentration in the range from. When the microbioassay media containing leucine were incubated for 6 h with L. mesenteroides and introduced into the lactate sensor system, a relationship between the current decrease and the leucine concentration was linear over the range of The current decrease was reproducible within 1% when the media containing of leucine was used. The electrochemical system was applied to the analysis of leucine in human sera. The results obtained by the present method were in good agreement with those obtained by an amino acid autoanalyzer (6 experiments, leucine concentrations in human sera; correlation coefficient; 0,98).
UR - http://www.scopus.com/inward/record.url?scp=85001284004&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85001284004&partnerID=8YFLogxK
U2 - 10.1246/nikkashi.1980.1537
DO - 10.1246/nikkashi.1980.1537
M3 - Article
VL - 1980
SP - 1537
EP - 1541
JO - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
JF - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
SN - 0369-4577
IS - 10
ER -