Microbioassay of phenylalanine in blood sera with a lactate electrode

Isao Karube, Tadashi Matsunaga, Nobuaki Teraoka, Shuichi Suzuki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Microbioassay of phenylalanine in sera is carried out by incubation with Leuconostoc mesenteroides (6 h) followed by use of a lactate electrode (immobilized lactate oxidase and an oxygen electrode) to measure the lactate produced. A linear relationship is obtained between the current decrease and the phenylalanine concentration between 0.75 and 6 × 10-7g ml-1 , with a standard deviation of 1.7 × 10-8 g ml-1 at the 3 × 10-7 g ml-1 level.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalAnalytica Chimica Acta
Volume119
Issue number2
DOIs
Publication statusPublished - 1980
Externally publishedYes

Fingerprint

lactate 2-monooxygenase
Phenylalanine
serum
Lactic Acid
Electrodes
electrode
Blood
blood
Serum
incubation
Oxygen
oxygen
Leuconostoc mesenteroides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy

Cite this

Microbioassay of phenylalanine in blood sera with a lactate electrode. / Karube, Isao; Matsunaga, Tadashi; Teraoka, Nobuaki; Suzuki, Shuichi.

In: Analytica Chimica Acta, Vol. 119, No. 2, 1980, p. 271-276.

Research output: Contribution to journalArticle

Karube, Isao ; Matsunaga, Tadashi ; Teraoka, Nobuaki ; Suzuki, Shuichi. / Microbioassay of phenylalanine in blood sera with a lactate electrode. In: Analytica Chimica Acta. 1980 ; Vol. 119, No. 2. pp. 271-276.
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