Microbiochemical systems for enzymatic reactions

Kazuo Hosokawa; Teruo Fujii; Shuichi Shoji; Akira Yotumoto; Takahiko Nojima; Isao Endo

Microbiochemical systems for enzymatic reactions

Kazuo Hosokawa^*, Teruo Fujii, Shuichi Shoji, Akira Yotumoto, Takahiko Nojima, Isao Endo

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper discusses microfabricated structures for biochemical studies. We built microreactors using conventional MEMS (microelectromechanical systems) techniques-silicon anisotropic etching and glass-silicon anodic bonding. In the microreactors, luciferin-luciferase reaction was conducted as an example of enzymatic reactions. Firefly luciferase catalyzes oxidation of luciferin. Luminescence induced by this reaction was photographed on a extra-high speed film. Diffusion-based mixing in the microreactors was dearly visualized. Such information would be helpful for developing more complicated microbiochemical systems, can be detected easily because of its luminescence.

Original language	English
Number of pages	1
Publication status	Published - 1997 Dec 1
Externally published	Yes
Event	Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97 - Tokyo, Jpn Duration: 1997 Jun 16 → 1997 Jun 20

Other

Other	Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97
City	Tokyo, Jpn
Period	97/6/16 → 97/6/20

ASJC Scopus subject areas

Control and Systems Engineering
Software
Computer Science Applications
Electrical and Electronic Engineering

Cite this

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title = "Microbiochemical systems for enzymatic reactions",

abstract = "This paper discusses microfabricated structures for biochemical studies. We built microreactors using conventional MEMS (microelectromechanical systems) techniques-silicon anisotropic etching and glass-silicon anodic bonding. In the microreactors, luciferin-luciferase reaction was conducted as an example of enzymatic reactions. Firefly luciferase catalyzes oxidation of luciferin. Luminescence induced by this reaction was photographed on a extra-high speed film. Diffusion-based mixing in the microreactors was dearly visualized. Such information would be helpful for developing more complicated microbiochemical systems, can be detected easily because of its luminescence.",

author = "Kazuo Hosokawa and Teruo Fujii and Shuichi Shoji and Akira Yotumoto and Takahiko Nojima and Isao Endo",

year = "1997",

month = dec,

day = "1",

language = "English",

note = "Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97 ; Conference date: 16-06-1997 Through 20-06-1997",

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TY - CONF

T1 - Microbiochemical systems for enzymatic reactions

AU - Hosokawa, Kazuo

AU - Fujii, Teruo

AU - Shoji, Shuichi

AU - Yotumoto, Akira

AU - Nojima, Takahiko

AU - Endo, Isao

PY - 1997/12/1

Y1 - 1997/12/1

N2 - This paper discusses microfabricated structures for biochemical studies. We built microreactors using conventional MEMS (microelectromechanical systems) techniques-silicon anisotropic etching and glass-silicon anodic bonding. In the microreactors, luciferin-luciferase reaction was conducted as an example of enzymatic reactions. Firefly luciferase catalyzes oxidation of luciferin. Luminescence induced by this reaction was photographed on a extra-high speed film. Diffusion-based mixing in the microreactors was dearly visualized. Such information would be helpful for developing more complicated microbiochemical systems, can be detected easily because of its luminescence.

AB - This paper discusses microfabricated structures for biochemical studies. We built microreactors using conventional MEMS (microelectromechanical systems) techniques-silicon anisotropic etching and glass-silicon anodic bonding. In the microreactors, luciferin-luciferase reaction was conducted as an example of enzymatic reactions. Firefly luciferase catalyzes oxidation of luciferin. Luminescence induced by this reaction was photographed on a extra-high speed film. Diffusion-based mixing in the microreactors was dearly visualized. Such information would be helpful for developing more complicated microbiochemical systems, can be detected easily because of its luminescence.

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M3 - Paper

AN - SCOPUS:0031345077

T2 - Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97

Y2 - 16 June 1997 through 20 June 1997

ER -

Microbiochemical systems for enzymatic reactions

Abstract

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ASJC Scopus subject areas

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