Microbiochemical systems for enzymatic reactions

Kazuo Hosokawa, Teruo Fujii, Shuichi Shoji, Akira Yotumoto, Takahiko Nojima, Isao Endo

Research output: Contribution to conferencePaper

Abstract

This paper discusses microfabricated structures for biochemical studies. We built microreactors using conventional MEMS (microelectromechanical systems) techniques-silicon anisotropic etching and glass-silicon anodic bonding. In the microreactors, luciferin-luciferase reaction was conducted as an example of enzymatic reactions. Firefly luciferase catalyzes oxidation of luciferin. Luminescence induced by this reaction was photographed on a extra-high speed film. Diffusion-based mixing in the microreactors was dearly visualized. Such information would be helpful for developing more complicated microbiochemical systems, can be detected easily because of its luminescence.

Original languageEnglish
Number of pages1
Publication statusPublished - 1997 Dec 1
EventProceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97 - Tokyo, Jpn
Duration: 1997 Jun 161997 Jun 20

Other

OtherProceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97
CityTokyo, Jpn
Period97/6/1697/6/20

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ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Hosokawa, K., Fujii, T., Shoji, S., Yotumoto, A., Nojima, T., & Endo, I. (1997). Microbiochemical systems for enzymatic reactions. Paper presented at Proceedings of the 1997 1st IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM'97, Tokyo, Jpn, .