Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome

Daichi Urushiyama, Wataru Suda, Eriko Ohnishi, Ryota Araki, Chihiro Kiyoshima, Masamitsu Kurakazu, Ayako Sanui, Fusanori Yotsumoto, Masaharu Murata, Kazuki Nabeshima, Shin'ichiro Yasunaga, Shigeru Saito, Makoto Nomiyama, Masahira Hattori, Shingo Miyamoto, Kenichiro Hata

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Chorioamnionitis (CAM), an inflammation of the foetal membranes due to infection, is associated with preterm birth and poor perinatal prognosis. The present study aimed to determine whether CAM can be diagnosed prior to delivery based on the bacterial composition of the amniotic fluid (AF). AF samples from 79 patients were classified according to placental inflammation: Stage III (n = 32), CAM; Stage II (n = 27), chorionitis; Stage 0-I (n = 20), sub-chorionitis or no neutrophil infiltration; and normal AF in early pregnancy (n = 18). Absolute quantification and sequencing of 16S rDNA showed that in Stage III, the 16S rDNA copy number was significantly higher and the α-diversity index lower than those in the other groups. In principal coordinate analysis, Stage III formed a separate cluster from Stage 0-I, normal AF, and blank. Forty samples were classified as positive for microbiomic CAM (miCAM) defined by the presence of 11 bacterial species that were found to be significantly associated with CAM and some parameters of perinatal prognosis. The diagnostic accuracy for CAM according to miCAM was: sensitivity, approximately 94%, and specificity, 79-87%. Our findings indicate the possibility of predicting CAM prior to delivery based on the AF microbiome profile.

    Original languageEnglish
    Article number12171
    JournalScientific Reports
    Volume7
    Issue number1
    DOIs
    Publication statusPublished - 2017 Dec 1

    Fingerprint

    Chorioamnionitis
    Microbiota
    Amniotic Fluid
    Biomarkers
    Ribosomal DNA
    Inflammation
    Extraembryonic Membranes
    Neutrophil Infiltration
    Premature Birth
    Pregnancy
    Infection

    ASJC Scopus subject areas

    • General

    Cite this

    Urushiyama, D., Suda, W., Ohnishi, E., Araki, R., Kiyoshima, C., Kurakazu, M., ... Hata, K. (2017). Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome. Scientific Reports, 7(1), [12171]. https://doi.org/10.1038/s41598-017-11699-8

    Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome. / Urushiyama, Daichi; Suda, Wataru; Ohnishi, Eriko; Araki, Ryota; Kiyoshima, Chihiro; Kurakazu, Masamitsu; Sanui, Ayako; Yotsumoto, Fusanori; Murata, Masaharu; Nabeshima, Kazuki; Yasunaga, Shin'ichiro; Saito, Shigeru; Nomiyama, Makoto; Hattori, Masahira; Miyamoto, Shingo; Hata, Kenichiro.

    In: Scientific Reports, Vol. 7, No. 1, 12171, 01.12.2017.

    Research output: Contribution to journalArticle

    Urushiyama, D, Suda, W, Ohnishi, E, Araki, R, Kiyoshima, C, Kurakazu, M, Sanui, A, Yotsumoto, F, Murata, M, Nabeshima, K, Yasunaga, S, Saito, S, Nomiyama, M, Hattori, M, Miyamoto, S & Hata, K 2017, 'Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome', Scientific Reports, vol. 7, no. 1, 12171. https://doi.org/10.1038/s41598-017-11699-8
    Urushiyama D, Suda W, Ohnishi E, Araki R, Kiyoshima C, Kurakazu M et al. Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome. Scientific Reports. 2017 Dec 1;7(1). 12171. https://doi.org/10.1038/s41598-017-11699-8
    Urushiyama, Daichi ; Suda, Wataru ; Ohnishi, Eriko ; Araki, Ryota ; Kiyoshima, Chihiro ; Kurakazu, Masamitsu ; Sanui, Ayako ; Yotsumoto, Fusanori ; Murata, Masaharu ; Nabeshima, Kazuki ; Yasunaga, Shin'ichiro ; Saito, Shigeru ; Nomiyama, Makoto ; Hattori, Masahira ; Miyamoto, Shingo ; Hata, Kenichiro. / Microbiome profile of the amniotic fluid as a predictive biomarker of perinatal outcome. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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