Microchannel array for long term monitoring of multicellular filamentous cyanobacterium with controlled perfusion culture

Risa Kajiyama, Junichi Ishihara, Kentaro Kawai, Hideo Iwasaki, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We designed and fabricated a microfluidic system, which has 128 microchannels for observation of muliticellular filamentous cyanobacteria, Anabaena sp. PCC 7120, under microscope for a long term with controlled perfusion culture. Using automated programming, we were able to monitor cell differentiation and growth of many filaments at the same time in this device. We are able to inject medium or reagent individually into each channel at any time during the experiment. Since more than 100 different filaments were cultured for 96 hours continuously, we succeeded in observing their morphogenesis clearly.

    Original languageEnglish
    Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
    Pages819-821
    Number of pages3
    Volume2
    Publication statusPublished - 2011
    Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA
    Duration: 2011 Oct 22011 Oct 6

    Other

    Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
    CitySeattle, WA
    Period11/10/211/10/6

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    Keywords

    • Cell differentiation
    • Culture bacterium
    • Cyanobacterium
    • Microfluidic

    ASJC Scopus subject areas

    • Control and Systems Engineering

    Cite this

    Kajiyama, R., Ishihara, J., Kawai, K., Iwasaki, H., & Shoji, S. (2011). Microchannel array for long term monitoring of multicellular filamentous cyanobacterium with controlled perfusion culture. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 819-821)