Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples

Tae Kyu Lim, Hiroko Ohta, Tadashi Matsunaga

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody-antigen complexes were separated from free Fc-conjugated IgG antibody (Fc-IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200-2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.

Original languageEnglish
Pages (from-to)3316-3321
Number of pages6
JournalAnalytical Chemistry
Volume75
Issue number14
DOIs
Publication statusPublished - 2003 Jul 15
Externally publishedYes

Fingerprint

Cation Exchange Resins
Histamine
Assays
Blood
Immunoglobulin G
Antibodies
Histamine Antagonists
Antigen-Antibody Complex
Proteins
ferrocenecarboxylic acid

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples. / Lim, Tae Kyu; Ohta, Hiroko; Matsunaga, Tadashi.

In: Analytical Chemistry, Vol. 75, No. 14, 15.07.2003, p. 3316-3321.

Research output: Contribution to journalArticle

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