Microfabricated reactor for cell-free protein synthesis

Teruo Fujii, Kazuo Hosokawa, Takahiko Nojima, Shuichi Shoji, Akira Yotsumoto, Isao Endo

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Cell-free protein synthesis is one of the most promising techniques for discovery and production of novel biochemical compounds which are useful for medical and biological applications. MEMS (Microelectromechanical Systems) technology enables us to fabricate microscale reactor systems for protein synthesis. In this paper, we show that the target protein (polyphenylalanine) can be successfully synthesized in the fabricated reactor according to given mRNA templates (polyuridic acid) as the simplest cell-free protein synthesis system. Since the microfabrication is a suitable method to integrated such reactors in a massively parallel structure, the integrated version of the system can be a powerful tool for drug discovery, preparation of combinatorial peptide libraries, etc.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Pages2572-2574
Number of pages3
Volume6
Publication statusPublished - 1997
Externally publishedYes
EventProceedings of the 1997 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Chicago, IL, USA
Duration: 1997 Oct 301997 Nov 2

Other

OtherProceedings of the 1997 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CityChicago, IL, USA
Period97/10/3097/11/2

Fingerprint

Proteins
Peptide Library
Microfabrication
Peptides
MEMS
Messenger RNA
Acids
Drug Discovery
polyphenylalanine

ASJC Scopus subject areas

  • Bioengineering

Cite this

Fujii, T., Hosokawa, K., Nojima, T., Shoji, S., Yotsumoto, A., & Endo, I. (1997). Microfabricated reactor for cell-free protein synthesis. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 6, pp. 2572-2574). IEEE.

Microfabricated reactor for cell-free protein synthesis. / Fujii, Teruo; Hosokawa, Kazuo; Nojima, Takahiko; Shoji, Shuichi; Yotsumoto, Akira; Endo, Isao.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 6 IEEE, 1997. p. 2572-2574.

Research output: Chapter in Book/Report/Conference proceedingChapter

Fujii, T, Hosokawa, K, Nojima, T, Shoji, S, Yotsumoto, A & Endo, I 1997, Microfabricated reactor for cell-free protein synthesis. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 6, IEEE, pp. 2572-2574, Proceedings of the 1997 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Chicago, IL, USA, 97/10/30.
Fujii T, Hosokawa K, Nojima T, Shoji S, Yotsumoto A, Endo I. Microfabricated reactor for cell-free protein synthesis. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 6. IEEE. 1997. p. 2572-2574
Fujii, Teruo ; Hosokawa, Kazuo ; Nojima, Takahiko ; Shoji, Shuichi ; Yotsumoto, Akira ; Endo, Isao. / Microfabricated reactor for cell-free protein synthesis. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 6 IEEE, 1997. pp. 2572-2574
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