Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

M. Haneoka, Y. Shirasaki, H. Sugino, Tetsushi Sekiguchi, Donghyun Yoon, R. Iizuka, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1773-1775
Number of pages3
Volume3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Sorting
Microfluidics
Hydrogels
Semiconductor quantum dots
Sol-gels
DNA
Molecules
Biomolecules
Gelation
Flow control
Laser beams
Polymers

Keywords

  • Microfluidics
  • On-chip sorter
  • Quantum dot
  • Thermo-reversible gelation polymer

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Haneoka, M., Shirasaki, Y., Sugino, H., Sekiguchi, T., Yoon, D., Iizuka, R., ... Funatsu, T. (2011). Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 1773-1775)

Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. / Haneoka, M.; Shirasaki, Y.; Sugino, H.; Sekiguchi, Tetsushi; Yoon, Donghyun; Iizuka, R.; Shoji, Shuichi; Funatsu, T.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1773-1775.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Haneoka, M, Shirasaki, Y, Sugino, H, Sekiguchi, T, Yoon, D, Iizuka, R, Shoji, S & Funatsu, T 2011, Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1773-1775, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, 11/10/2.
Haneoka M, Shirasaki Y, Sugino H, Sekiguchi T, Yoon D, Iizuka R et al. Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3. 2011. p. 1773-1775
Haneoka, M. ; Shirasaki, Y. ; Sugino, H. ; Sekiguchi, Tetsushi ; Yoon, Donghyun ; Iizuka, R. ; Shoji, Shuichi ; Funatsu, T. / Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. pp. 1773-1775
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