Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

M. Haneoka, Y. Shirasaki, H. Sugino, T. Sekiguchi, D. H. Yoon, R. Iizuka, S. Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1773-1775
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Microfluidics
  • On-chip sorter
  • Quantum dot
  • Thermo-reversible gelation polymer

ASJC Scopus subject areas

  • Control and Systems Engineering

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    Haneoka, M., Shirasaki, Y., Sugino, H., Sekiguchi, T., Yoon, D. H., Iizuka, R., Shoji, S., & Funatsu, T. (2011). Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1773-1775). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).