TY - GEN
T1 - Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition
AU - Haneoka, M.
AU - Shirasaki, Y.
AU - Sugino, H.
AU - Sekiguchi, T.
AU - Yoon, D. H.
AU - Iizuka, R.
AU - Shoji, S.
AU - Funatsu, T.
N1 - Funding Information:
The authors thank Osamu Ohara (RIKEN) for providing the DNAs of actin and GAPDH, and Mai Yamagishi (RIKEN), Kazuhiro Hiyama, and Ryosuke Jikuya for assistance in the early stages of the experiment. This research was partly supported by Sentan, JST and by Grants-in-Aid for Scientific Research (A) ( 19206046 to S.S.) and for Young Scientists (B) ( 20-710103 to Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan . This research was also supported by the Japan Society for the Promotion of Science (JSPS) through its Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program). H.S. ( 20-9830 ) and T.A. ( 20-10635 ) were the recipients of a Research Fellowship from the Japan Society for the Promotion of Science for Young Scientists . Appendix A
PY - 2011
Y1 - 2011
N2 - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
AB - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
KW - Microfluidics
KW - On-chip sorter
KW - Quantum dot
KW - Thermo-reversible gelation polymer
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M3 - Conference contribution
AN - SCOPUS:84883822304
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1773
EP - 1775
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -