Abstract
A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 1773-1775 |
| Number of pages | 3 |
| Volume | 3 |
| Publication status | Published - 2011 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA Duration: 2011 Oct 2 → 2011 Oct 6 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| City | Seattle, WA |
| Period | 11/10/2 → 11/10/6 |
Fingerprint
Keywords
- Microfluidics
- On-chip sorter
- Quantum dot
- Thermo-reversible gelation polymer
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. / Haneoka, M.; Shirasaki, Y.; Sugino, H.; Sekiguchi, Tetsushi; Yoon, Donghyun; Iizuka, R.; Shoji, Shuichi; Funatsu, T.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1773-1775.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition
AU - Haneoka, M.
AU - Shirasaki, Y.
AU - Sugino, H.
AU - Sekiguchi, Tetsushi
AU - Yoon, Donghyun
AU - Iizuka, R.
AU - Shoji, Shuichi
AU - Funatsu, T.
PY - 2011
Y1 - 2011
N2 - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
AB - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
KW - Microfluidics
KW - On-chip sorter
KW - Quantum dot
KW - Thermo-reversible gelation polymer
UR - http://www.scopus.com/inward/record.url?scp=84883822304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883822304&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883822304
SN - 9781618395955
VL - 3
SP - 1773
EP - 1775
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -