TY - GEN
T1 - Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition
AU - Haneoka, M.
AU - Shirasaki, Y.
AU - Sugino, H.
AU - Sekiguchi, T.
AU - Yoon, D. H.
AU - Iizuka, R.
AU - Shoji, S.
AU - Funatsu, T.
N1 - Funding Information:
The authors thank Osamu Ohara (RIKEN) for providing the DNAs of actin and GAPDH, and Mai Yamagishi (RIKEN), Kazuhiro Hiyama, and Ryosuke Jikuya for assistance in the early stages of the experiment. This research was partly supported by Sentan, JST and by Grants-in-Aid for Scientific Research (A) ( 19206046 to S.S.) and for Young Scientists (B) ( 20-710103 to Y.S.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan . This research was also supported by the Japan Society for the Promotion of Science (JSPS) through its Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program). H.S. ( 20-9830 ) and T.A. ( 20-10635 ) were the recipients of a Research Fellowship from the Japan Society for the Promotion of Science for Young Scientists . Appendix A
Funding Information:
Mai Haneoka received her bachelor of pharmacology from the Faculty of Pharmaceutical Sciences, the University of Tokyo, Japan in 2010. She has been a graduate student of the Graduate School of Pharmaceutical Sciences, The University of Tokyo. Yoshitaka Shirasaki received his BS, MS and PhD degrees from Waseda University, Tokyo, Japan in 2002, 2004 and 2007, respectively. He was a researcher at the Kazusa DNA Research Institute in 2007. Since 2009, he has been a researcher at the Research Center for Allergy and Immunology, RIKEN. Hirokazu Sugino received his MS degree from the Graduate School of Science and Technology, Waseda University in 2006 and his PhD degree from the Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2009. He was a postdoctoral fellow of The University of Tokyo. Tokihiko Aoki received his ME degree from the Graduate School of Science and Technology, Waseda University in 2008. Takahiro Arakawa received his BE, ME and PhD degrees from Waseda University in 2002, 2004 and 2007, respectively. He was a postdoctoral fellow of The University of Tokyo in 2008. Since 2009, he has been an assistant professor at the Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University. Kazuto Ozaki received his ME degree from the Graduate School of Science and Technology, Waseda University, Japan, in 2010. Dong Hyun Yoon received his ME degree in the School of Mechanical Engineering from the Pusan National University, Busan, Korea, in 2008. He joined in Waseda University as a PhD candidate in the same year. His current research topic for thesis is the control of multiphase microfluidics. Noriyuki Ishii received his PhD degree from Tokyo Institute of Technology in 1992. He was a researcher at the Frontier Research Program, RIKEN, and a senior researcher at the Natl. Inst. of Bioscience and Human-Technology, and at the Biological Information Research Center, AIST. During this period, he was a visiting researcher at New York Univ. Medical Center, Lawrence Berkeley Natl. Lab., Univ. of California at Berkeley, and a specialist at the Bio-Industry Division, METI. Since 2010, he has been a senior researcher at the Biomedical Research Inst., AIST, and since 2009, a visiting associate professor at Tokyo Univ. of Agriculture and Technology. Ryo Iizuka received his BE and PhD degrees from Tokyo University of Agriculture and Technology (TUAT) in 2001 and 2004. He was a postdoctoral fellow at TUAT in 2004–2005, and worked as an assistant professor at TUAT in 2005–2006. From 2006 to 2009, he was the recipient of a Research Fellowship from the Japan Society for the Promotion of Science for Young Scientists. He has been an assistant professor at the Graduate School of Pharmaceutical Sciences, The University of Tokyo since 2009. Shuichi Shoji received his PhD degree in engineering from Tohoku University, Sendai, Japan. From 1992 to 1998, he was a research associate and an associate professor at Tohoku University. From 1992 to 1997, he was an associate professor at Waseda University. Since 1997, he has been a professor of electronics, information and communication engineering of Waseda University. Takashi Funatsu received his BS, MS and PhD degrees from Waseda University in 1982, 1984 and 1988, respectively. He was an associate professor at Waseda University in 1997. Since 2004, he has been a professor of bioanalytical chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo.
PY - 2011
Y1 - 2011
N2 - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
AB - A microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer (TGP). Using the developed sorter, Qdot-labeled actin DNA was separated from unwanted DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nanometer-sized particles of Qdots.
KW - Microfluidics
KW - On-chip sorter
KW - Quantum dot
KW - Thermo-reversible gelation polymer
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M3 - Conference contribution
AN - SCOPUS:84883822304
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1773
EP - 1775
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -