Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Donghyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji, Takashi Funatsu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in ∼10 μm channels. Single Qdots with diameters of ∼10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume159
Issue number1
DOIs
Publication statusPublished - 2011 Nov 28

Fingerprint

Hydrogel
classifying
Sorting
Microfluidics
Hydrogels
Semiconductor quantum dots
Sol-gels
DNA
deoxyribonucleic acid
quantum dots
gels
Molecules
Flow control
Actins
molecules
Glyceraldehyde-3-Phosphate Dehydrogenases
Biomolecules
Gelation
Viruses
Flow velocity

Keywords

  • DNA
  • Microfluidics
  • On-chip sorter
  • Quantum dot
  • Separation
  • Thermo-reversible gelation polymer

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. / Haneoka, Mai; Shirasaki, Yoshitaka; Sugino, Hirokazu; Aoki, Tokihiko; Arakawa, Takahiro; Ozaki, Kazuto; Yoon, Donghyun; Ishii, Noriyuki; Iizuka, Ryo; Shoji, Shuichi; Funatsu, Takashi.

In: Sensors and Actuators, B: Chemical, Vol. 159, No. 1, 28.11.2011, p. 314-320.

Research output: Contribution to journalArticle

Haneoka, Mai ; Shirasaki, Yoshitaka ; Sugino, Hirokazu ; Aoki, Tokihiko ; Arakawa, Takahiro ; Ozaki, Kazuto ; Yoon, Donghyun ; Ishii, Noriyuki ; Iizuka, Ryo ; Shoji, Shuichi ; Funatsu, Takashi. / Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition. In: Sensors and Actuators, B: Chemical. 2011 ; Vol. 159, No. 1. pp. 314-320.
@article{d11931e3ab584a4fb98a1f84ae9b1798,
title = "Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition",
abstract = "The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in ∼10 μm channels. Single Qdots with diameters of ∼10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots.",
keywords = "DNA, Microfluidics, On-chip sorter, Quantum dot, Separation, Thermo-reversible gelation polymer",
author = "Mai Haneoka and Yoshitaka Shirasaki and Hirokazu Sugino and Tokihiko Aoki and Takahiro Arakawa and Kazuto Ozaki and Donghyun Yoon and Noriyuki Ishii and Ryo Iizuka and Shuichi Shoji and Takashi Funatsu",
year = "2011",
month = "11",
day = "28",
doi = "10.1016/j.snb.2011.06.043",
language = "English",
volume = "159",
pages = "314--320",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol-gel transition

AU - Haneoka, Mai

AU - Shirasaki, Yoshitaka

AU - Sugino, Hirokazu

AU - Aoki, Tokihiko

AU - Arakawa, Takahiro

AU - Ozaki, Kazuto

AU - Yoon, Donghyun

AU - Ishii, Noriyuki

AU - Iizuka, Ryo

AU - Shoji, Shuichi

AU - Funatsu, Takashi

PY - 2011/11/28

Y1 - 2011/11/28

N2 - The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in ∼10 μm channels. Single Qdots with diameters of ∼10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots.

AB - The fluorescence-activated cell sorter instrument has contributed significantly to life sciences. However, this instrument has limitations including the inability to detect and hence sort nanometer-sized particles such as quantum dots (Qdots) and virus particles. Here, a microfluidic device for analyzing and sorting nanometer-sized particles has been developed. To achieve sensitive detection, the sample flow was hydrodynamically sheathed and effectively excited with a focused laser beam. Flow control was performed by a sol-gel transition of a thermoreversible gelation polymer. This flow control approach enabled us to restrict the lateral diffusion of nanometer-sized particles and to sort the particles in ∼10 μm channels. Single Qdots with diameters of ∼10 nm were detected at a linear flow velocity of about 4 mm/s, and the Qdots were successfully sorted with the sorting system. Using the developed sorter in an application, Qdot-labeled actin DNA was separated from unwanted glyceraldehyde-3-phosphate dehydrogenase DNA, and the purity of Qdot-labeled actin DNA increased following the sorting. This study represents the first example of active separation of biomolecules labeled with single 10 nm-sized particles of Qdots.

KW - DNA

KW - Microfluidics

KW - On-chip sorter

KW - Quantum dot

KW - Separation

KW - Thermo-reversible gelation polymer

UR - http://www.scopus.com/inward/record.url?scp=80052329244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052329244&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2011.06.043

DO - 10.1016/j.snb.2011.06.043

M3 - Article

VL - 159

SP - 314

EP - 320

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 1

ER -