Microfluidic alignment system for single cell manipulation and culture

Takahiro Arakawa, Naoya Takeda, Yoshikuni Edagawa, Yoshinori Yamaguchi, S. Shoji

Research output: Contribution to conferencePaperpeer-review

Abstract

Microfluidic alignment system for single cell manipulation and culture was developed to investigate the differences between individual cells in cell biology. We describe a microfluidic manipulation system that consists of a main channel, a pressure balancing channel (PBC), 3 μm drain channels and semicircle micropockets of 100 μm in diameter, for single cell capture, cell release and cell culture. Fundamental single cell manipulation was obtained by simple flow rate, the ratio between the cells that passed though the injected microchannel and captured cell were about 72%. Multiplication from the single cell in the micropocket was confirmed up to the third generation. This microfluidic system realized noninvasive capture of living single cells, and long-term culture up to 6 days. And this system contributes to the elucidation of cellular nature at the single cell level.

Original languageEnglish
Pages1855-1857
Number of pages3
Publication statusPublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Cell rupture
  • Single cell culture
  • Single cell manipulation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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