Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki, Jun Mizuno, Takashi Kasahara, Tomohiko Edura, Eri Kunisawa, Ryoichi Ishimatsu, Shigeyuki Matsunami, Toshihiko Imato, Chihaya Adachi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

Original languageEnglish
Title of host publicationMEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages108-111
Number of pages4
ISBN (Print)9781479935086
DOIs
Publication statusPublished - 2014 Jan 1
Event27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
Duration: 2014 Jan 262014 Jan 30

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
CountryUnited States
CitySan Francisco, CA
Period14/1/2614/1/30

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Tsuwaki, M., Mizuno, J., Kasahara, T., Edura, T., Kunisawa, E., Ishimatsu, R., Matsunami, S., Imato, T., Adachi, C., & Shoji, S. (2014). Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 108-111). [6765585] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765585