Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system

Yuyang Lee, Ryo Sudo, Tomoya Komatsu, Norihisa Miki, Toshihiro Mitaka, Mariko Ikeda, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Reconstruction of hepato-biliary networks is very important to maintaining liver organoids in vitro, since the accumulation of bile has been proven to be toxic to hepatocytes, especially in long-term culture. We have developed culture methods for reconstructing bile canaliculi (BC) and bile ducts (BDs) formed by small hepatocytes (SHs) which are hepatic progenitor cells, and biliary epithelial cells (BECs), respectively. To study the mechanism of biliary excretion, we need to establish a co-culture system for SHs and BECs. A limitation of conventional culture methods in investigating the mechanisms of hepato-biliary connections is the difficulty to observe the dynamic interactions between SHs and BECs in culture. In addition, patterned constructs do not form in conventional cultures due to the lack of organization of cells. A system that allows more complex dynamics between SHs and BECs to occur is needed in order to investigate the interactions between these cell types. Here, we present an approach called Microfluidic Hydrostatic Deposition Patterning (MHDP) that patterns SH and BEC colonies with defined shape and position, which promotes productive interactions between the cells and eventually induce the formation of heterogeneous tissues integration which is thought to be a prototype of complete biliary networks.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
Pages10-15
Number of pages6
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya
Duration: 2011 Nov 62011 Nov 9

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CityNagoya
Period11/11/611/11/9

Fingerprint

Microfluidics
Epithelial Cells
Hepatocytes
Liver
Ducts
Tissue

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

Cite this

Lee, Y., Sudo, R., Komatsu, T., Miki, N., Mitaka, T., Ikeda, M., & Tanishita, K. (2012). Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 10-15). [6102148] https://doi.org/10.1109/MHS.2011.6102148

Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system. / Lee, Yuyang; Sudo, Ryo; Komatsu, Tomoya; Miki, Norihisa; Mitaka, Toshihiro; Ikeda, Mariko; Tanishita, Kazuo.

2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 10-15 6102148.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lee, Y, Sudo, R, Komatsu, T, Miki, N, Mitaka, T, Ikeda, M & Tanishita, K 2012, Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system. in 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"., 6102148, pp. 10-15, 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation, Nagoya, 11/11/6. https://doi.org/10.1109/MHS.2011.6102148
Lee Y, Sudo R, Komatsu T, Miki N, Mitaka T, Ikeda M et al. Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 10-15. 6102148 https://doi.org/10.1109/MHS.2011.6102148
Lee, Yuyang ; Sudo, Ryo ; Komatsu, Tomoya ; Miki, Norihisa ; Mitaka, Toshihiro ; Ikeda, Mariko ; Tanishita, Kazuo. / Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system. 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. pp. 10-15
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