Microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme

Daiki Tanaka, Wataru Kawakubo, Erika Tsuda, Yuya Mitsumoto, Donghyun Yoon, Tetsushi Sekiguchi, Takashiro Akitsu, Shuichi Shoji

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Efficient microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme was achieved. The reaction conditions for the delicate protein were controlled precisely with a separation barrier in the microfluidic device. The microfluidic method has the following advantages over the conventional method: a remarkable reduction in the reaction time from 4.5 h to less than 1 s for the synthesis of Mn(ii) and Co(ii) complexes, a reduction in the reaction temperature from 40 to 23 °C, and no need for an N2 atmosphere because all the reagents are isolated from the air. A three-fold yield improvement was achieved for Mn(ii) and Co(ii) complexes containing lysozyme compared with conventional synthesis.

Original languageEnglish
Pages (from-to)81862-81868
Number of pages7
JournalRSC Advances
Volume6
Issue number85
DOIs
Publication statusPublished - 2016

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Muramidase
Microfluidics
Enzymes
Proteins
Air
Mn-salen
Temperature

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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Microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme. / Tanaka, Daiki; Kawakubo, Wataru; Tsuda, Erika; Mitsumoto, Yuya; Yoon, Donghyun; Sekiguchi, Tetsushi; Akitsu, Takashiro; Shoji, Shuichi.

In: RSC Advances, Vol. 6, No. 85, 2016, p. 81862-81868.

Research output: Contribution to journalArticle

Tanaka, Daiki ; Kawakubo, Wataru ; Tsuda, Erika ; Mitsumoto, Yuya ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Akitsu, Takashiro ; Shoji, Shuichi. / Microfluidic synthesis of chiral salen Mn(ii) and Co(ii) complexes containing lysozyme. In: RSC Advances. 2016 ; Vol. 6, No. 85. pp. 81862-81868.
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AU - Yoon, Donghyun

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AU - Akitsu, Takashiro

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