Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip

Hiroaki Takehara, Katsuhisa Sakaguchi, Hidekazu Sekine, Teruo Okano, Tatsuya Shimizu

Research output: Contribution to journalArticle

Abstract

In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections between endothelial capillaries of heterogeneous sections (5–100 μm in diameter) and artificial plastic tubes or reservoirs (1–10 mm in diameter). Moreover, the micro-VB device can be installed in a standard 100 mm-diameter Petri dish. Endothelial networks in 3D engineered tissues were obtained by cellular self-assembly on the device, after co-culturing of human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs) in fibrin gel. Endothelial capillary connection between vascularized tissues and microfluidic channels, mimicking arteries and veins, was confirmed by perfusion of fluorescent microspheres. The micro-VB devices were compatible with the use of commercially available culture dishes and did not require the involvement of additional equipment. Thus, these micro-VB devices are expected to substantially improve the routine application of 3D tissue engineering to regenerative medicine.

Original languageEnglish
Article number9
JournalBiomedical Microdevices
Volume22
Issue number1
DOIs
Publication statusPublished - 2020 Mar 1

Keywords

  • Artificial vessels
  • Drug screening
  • Endothelial cells
  • Petri dish
  • Regenerative medicine
  • Vascularization

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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