TY - JOUR
T1 - Microparticle Responses to Aerobic Exercise and Meal Consumption in Healthy Men
AU - Highton, Patrick J.
AU - Goltz, Fernanda R.
AU - Martin, Naomi
AU - Stensel, David J.
AU - Thackray, Alice E.
AU - Bishop, Nicolette C.
N1 - Funding Information:
This research was supported by the NIHR Leicester Biomedical Research Centre. Current affiliation for Patrick Highton: Collaborations for Leadership in Applied Health and Research Care (CLAHRC) within the Diabetes Research Centre, University of Leicester, Leicester, United Kingdom. The authors would also like to thank Dr. Owen Davies for his advice and expertise in nanoparticle tracking analysis.
Funding Information:
This research was supported by the NIHR Leicester Biomedical Research Centre. Current affiliation for Patrick Highton: Collaborations for Leadership in Applied Health and Research Care (CLAHRC) within the Diabetes Research Centre, University of Leicester, Leicester, United Kingdom. The authors would also like to thank Dr. Owen Davies for his advice and expertise in nanoparticle tracking analysis.
Publisher Copyright:
© 2019 by the American College of Sports Medicine.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Purpose Microparticles (MP) are shed extracellular vesicles that express the prothrombotic tissue factor (TF). Aerobic exercise may reduce MP count and TF expression. This study investigated the impact of acute running or rest followed by standardized meal consumption on MP phenotypes and TF expression. Methods Fifteen males (age, 22.9 ± 3.3 yr; body mass, 81.9 ± 11.4 kg; VO2max, 54.9 ± 6.5 mL·kg-1·min-1; mean ± SD) completed 1 h of running (70% VO2max) or rest at 9:00 am and consumed a standardized meal (1170 kcal, 43% CHO, 17% PRO, 40% fat) at 10:45 am. Venous blood samples were taken at 9:00 am, 10:00 am, and 11:30 am. The MP concentration, diameter, phenotypes, and TF expression were assessed using nanoparticle tracking analysis and flow cytometry. Results Nanoparticle tracking analysis identified no changes in MP concentration or diameter in response to time or trial. Flow cytometry revealed total MP count increased from 9:00 am to 10:00 am (1.62 ± 2.28 to 1.74 ± 2.61 × 1010 L-1, P = 0.016, effect size (η2) = 0.105), but was unaffected by trial. TF+ platelet-derived MP % reduced from 9:00 am to 10:00 am (44.0% ± 21.2% to 21.5% ± 9.3%, P = 0.001, η2 = 0.582) after exercise only (control, 36.8% ± 18.2% to 34.9% ± 11.9%; P = 0.972). TF+ neutrophil-derived MP percentage reduced from 9:00 am to 11:30 am (42.3% ± 17.2% to 25.1% ± 14.9%; P = 0.048, η2 = 0.801) in the exercise trial only (control, 28.5% ± 15.7% to 32.2% ± 9.6%; P = 0.508). Conclusions Running induced a significant reduction in %TF+ platelet and neutrophil MP, suggesting a transient reduction in cardiovascular risk via reduced TF-stimulated thrombosis. This requires further investigation over longer periods in cardiovascular disease populations.
AB - Purpose Microparticles (MP) are shed extracellular vesicles that express the prothrombotic tissue factor (TF). Aerobic exercise may reduce MP count and TF expression. This study investigated the impact of acute running or rest followed by standardized meal consumption on MP phenotypes and TF expression. Methods Fifteen males (age, 22.9 ± 3.3 yr; body mass, 81.9 ± 11.4 kg; VO2max, 54.9 ± 6.5 mL·kg-1·min-1; mean ± SD) completed 1 h of running (70% VO2max) or rest at 9:00 am and consumed a standardized meal (1170 kcal, 43% CHO, 17% PRO, 40% fat) at 10:45 am. Venous blood samples were taken at 9:00 am, 10:00 am, and 11:30 am. The MP concentration, diameter, phenotypes, and TF expression were assessed using nanoparticle tracking analysis and flow cytometry. Results Nanoparticle tracking analysis identified no changes in MP concentration or diameter in response to time or trial. Flow cytometry revealed total MP count increased from 9:00 am to 10:00 am (1.62 ± 2.28 to 1.74 ± 2.61 × 1010 L-1, P = 0.016, effect size (η2) = 0.105), but was unaffected by trial. TF+ platelet-derived MP % reduced from 9:00 am to 10:00 am (44.0% ± 21.2% to 21.5% ± 9.3%, P = 0.001, η2 = 0.582) after exercise only (control, 36.8% ± 18.2% to 34.9% ± 11.9%; P = 0.972). TF+ neutrophil-derived MP percentage reduced from 9:00 am to 11:30 am (42.3% ± 17.2% to 25.1% ± 14.9%; P = 0.048, η2 = 0.801) in the exercise trial only (control, 28.5% ± 15.7% to 32.2% ± 9.6%; P = 0.508). Conclusions Running induced a significant reduction in %TF+ platelet and neutrophil MP, suggesting a transient reduction in cardiovascular risk via reduced TF-stimulated thrombosis. This requires further investigation over longer periods in cardiovascular disease populations.
KW - CARDIOVASCULAR RISK
KW - MICROPARTICLE PHENOTYPES
KW - RUNNING
KW - THROMBOTIC POTENTIAL
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U2 - 10.1249/MSS.0000000000001985
DO - 10.1249/MSS.0000000000001985
M3 - Article
C2 - 30889043
AN - SCOPUS:85070834144
SN - 0195-9131
VL - 51
SP - 1935
EP - 1943
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 9
ER -