MicroRNA expression profiling in skeletal muscle reveals different regulatory patterns in high and low responders to resistance training

Riki Ogasawara, Takayuki Akimoto, Tokushi Umeno, Shuji Sawada, Takafumi Hamaoka, Satoshi Fujita

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Large variability exists in muscle adaptive response to resistance exercise (RE) training between individuals. Recent studies have revealed a significant role for microRNAs (miRNAs) in skeletal muscle plasticity. In this study, we investigated how RE affects miRNA expression and whether the variability of muscle hypertrophy to RE training may be attributed to differential miRNA regulation in the skeletal muscle. To screen high and low responders to RE, we had 18 young men perform arm curl exercise training. After screening, all the men performed 12 wk of lower body RE training, but only the high or low responders participated in the acute RE test before training. Muscle biopsies were obtained from the vastus lateralis muscle at baseline, 3 h after acute RE, and after the training period. Total RNA was extracted from the skeletal muscle, and miRNA expression (800 miRNAs) was analyzed. RE training increased the cross-sectional area of the biceps brachii (-1.7-26.1%), quadriceps (2.2-16.8%), and hamstrings (1.6 -18.4%). Eighty-five and 102 miRNAs were differentially expressed after acute and chronic RE, respectively (P < 0.05). Seventeen miRNAs, especially 23b-3p, 26a-5p, 32-5p, 148b-3p, and 376a-3p, were differentially expressed at baseline, and 23 miRNAs, especially let-7a-5p, 95, 148a-3p, and 376a-3p, and 26 miRNAs, especially 30d-5p and 376a-3p, were differentially regulated after acute and chronic RE, respectively, in the skeletal muscle between high and low responders, indicating that the expression patterns of several miRNAs are altered by acute or chronic RE, and that miRNAs are involved in skeletal muscle adaptation to RE training.

Original languageEnglish
Pages (from-to)320-324
Number of pages5
JournalPhysiological Genomics
Volume48
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1
Externally publishedYes

Fingerprint

Resistance Training
MicroRNAs
Skeletal Muscle
Exercise
Muscles
Quadriceps Muscle
Exercise Test
Hypertrophy

Keywords

  • Comprehensive expression analysis
  • Exercise
  • Muscle hypertrophy

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

MicroRNA expression profiling in skeletal muscle reveals different regulatory patterns in high and low responders to resistance training. / Ogasawara, Riki; Akimoto, Takayuki; Umeno, Tokushi; Sawada, Shuji; Hamaoka, Takafumi; Fujita, Satoshi.

In: Physiological Genomics, Vol. 48, No. 4, 01.04.2016, p. 320-324.

Research output: Contribution to journalArticle

Ogasawara, Riki ; Akimoto, Takayuki ; Umeno, Tokushi ; Sawada, Shuji ; Hamaoka, Takafumi ; Fujita, Satoshi. / MicroRNA expression profiling in skeletal muscle reveals different regulatory patterns in high and low responders to resistance training. In: Physiological Genomics. 2016 ; Vol. 48, No. 4. pp. 320-324.
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