Microscale Visualization of Erythrocyte Deformation by Colliding with a Rigid Surface Using a High-Speed Impinging Jet

S. Wakasa, T. Yagi, Y. Akimoto, N. Tokunaga, K. Iwasaki, M. Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Erythrocyte deformation by colliding with a rigid surface using a high-speed impinging jet was studied with microfluidic techniques. We aim to investigate the relevance of colliding erythrocytes with hemolysis. A micro-channel chip was made of polydimenthyl-siloxane (PDMS), which comprised a T- and Y-shaped junction with a micro-nozzle and diffuser in order to attain a high-speed microflow with a jet velocity of m/s scale. A high-speed camera with a microscope imaged colliding erythrocytes by shadow imaging. Porcine erythrocyte with a hematocrit of 0.5 % in phosphate buffer saline was utilized. At the Y-junction, erythrocytes showed buckling due to an impulsive, longitudinal, compressive deformation. Such anomalous phenomena were not detected at the T-junction, where erythrocytes underwent sequential compressions as approaching the colliding surface. Erythrocyte after buckling showed a hazy membrane while being released from the colliding surface, suggesting the ejection of hemoglobin out of the pore on a membrane. Flow-induced hemolysis has been considered as a model of viscous shear stress and exposure time. From our data, however, it was suggested that hemolysis due to a high-speed impinging flow characterized by mechanical heart valve flows may arise as an impulsive failure of erythrocyte membrane upon collision.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages1422-1425
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: 2008 Dec 32008 Dec 6

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
CountrySingapore
Period08/12/308/12/6

Keywords

  • Hemolysis
  • erythrocyte
  • heart valve
  • microfluidics
  • turbulence

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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    Wakasa, S., Yagi, T., Akimoto, Y., Tokunaga, N., Iwasaki, K., & Umezu, M. (2009). Microscale Visualization of Erythrocyte Deformation by Colliding with a Rigid Surface Using a High-Speed Impinging Jet. In 13th International Conference on Biomedical Engineering - ICBME 2008 (pp. 1422-1425). (IFMBE Proceedings; Vol. 23). https://doi.org/10.1007/978-3-540-92841-6_351