Microscopic analysis of polymerization dynamics with individual actin filaments

Ikuko Fujiwara, Shin Takahashi, Hisashi Tadakuma, Takashi Funatsu, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    137 Citations (Scopus)

    Abstract

    The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

    Original languageEnglish
    Pages (from-to)666-673
    Number of pages8
    JournalNature Cell Biology
    Volume4
    Issue number9
    DOIs
    Publication statusPublished - 2002 Sep

    Fingerprint

    Actin Cytoskeleton
    Polymerization
    Stochastic Processes
    Actins

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T., & Ishiwata, S. (2002). Microscopic analysis of polymerization dynamics with individual actin filaments. Nature Cell Biology, 4(9), 666-673. https://doi.org/10.1038/ncb841

    Microscopic analysis of polymerization dynamics with individual actin filaments. / Fujiwara, Ikuko; Takahashi, Shin; Tadakuma, Hisashi; Funatsu, Takashi; Ishiwata, Shin'ichi.

    In: Nature Cell Biology, Vol. 4, No. 9, 09.2002, p. 666-673.

    Research output: Contribution to journalArticle

    Fujiwara, I, Takahashi, S, Tadakuma, H, Funatsu, T & Ishiwata, S 2002, 'Microscopic analysis of polymerization dynamics with individual actin filaments', Nature Cell Biology, vol. 4, no. 9, pp. 666-673. https://doi.org/10.1038/ncb841
    Fujiwara I, Takahashi S, Tadakuma H, Funatsu T, Ishiwata S. Microscopic analysis of polymerization dynamics with individual actin filaments. Nature Cell Biology. 2002 Sep;4(9):666-673. https://doi.org/10.1038/ncb841
    Fujiwara, Ikuko ; Takahashi, Shin ; Tadakuma, Hisashi ; Funatsu, Takashi ; Ishiwata, Shin'ichi. / Microscopic analysis of polymerization dynamics with individual actin filaments. In: Nature Cell Biology. 2002 ; Vol. 4, No. 9. pp. 666-673.
    @article{b0ff018ca1984531ab37111640e0d9aa,
    title = "Microscopic analysis of polymerization dynamics with individual actin filaments",
    abstract = "The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.",
    author = "Ikuko Fujiwara and Shin Takahashi and Hisashi Tadakuma and Takashi Funatsu and Shin'ichi Ishiwata",
    year = "2002",
    month = "9",
    doi = "10.1038/ncb841",
    language = "English",
    volume = "4",
    pages = "666--673",
    journal = "Nature Cell Biology",
    issn = "1465-7392",
    publisher = "Nature Publishing Group",
    number = "9",

    }

    TY - JOUR

    T1 - Microscopic analysis of polymerization dynamics with individual actin filaments

    AU - Fujiwara, Ikuko

    AU - Takahashi, Shin

    AU - Tadakuma, Hisashi

    AU - Funatsu, Takashi

    AU - Ishiwata, Shin'ichi

    PY - 2002/9

    Y1 - 2002/9

    N2 - The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

    AB - The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

    UR - http://www.scopus.com/inward/record.url?scp=0036713779&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0036713779&partnerID=8YFLogxK

    U2 - 10.1038/ncb841

    DO - 10.1038/ncb841

    M3 - Article

    VL - 4

    SP - 666

    EP - 673

    JO - Nature Cell Biology

    JF - Nature Cell Biology

    SN - 1465-7392

    IS - 9

    ER -