Microscopic analysis of polymerization dynamics with individual actin filaments

Ikuko Fujiwara, Shin Takahashi, Hisashi Tadakuma, Takashi Funatsu, Shin'ichi Ishiwata

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    143 Citations (Scopus)

    Abstract

    The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.

    Original languageEnglish
    Pages (from-to)666-673
    Number of pages8
    JournalNature Cell Biology
    Volume4
    Issue number9
    DOIs
    Publication statusPublished - 2002 Sep

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    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T., & Ishiwata, S. (2002). Microscopic analysis of polymerization dynamics with individual actin filaments. Nature Cell Biology, 4(9), 666-673. https://doi.org/10.1038/ncb841