Microscopic detection of thermogenesis in a single HeLa cell

Madoka Suzuki, Vadim Tseeb, Kotaro Oyama, Shin'ichi Ishiwata

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

We report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive fluorescent dye Europium (III) thenoyltrifluoroacetonate trihydrate). We found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca2+ influx from the extracellular space. The time delay inversely depended on extracellular [Ca2+], and the increase in temperature was suppressed when Ca2+-ATPases were blocked by thapsigargin. These observations strongly suggest that the enzymatic activity of Ca 2+-ATPases in endoplasmic reticulum leads to the heat production. This study has therefore paved the way for studying the thermogenesis at the single-cell level.

Original languageEnglish
JournalBiophysical Journal
Volume92
Issue number6
DOIs
Publication statusPublished - 2007 Mar

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Thermogenesis
HeLa Cells
Europium
Ionomycin
Temperature
Thapsigargin
Calcium-Transporting ATPases
Extracellular Space
Fluorescent Dyes
Endoplasmic Reticulum
Glass
Adenosine Triphosphatases

ASJC Scopus subject areas

  • Biophysics

Cite this

Microscopic detection of thermogenesis in a single HeLa cell. / Suzuki, Madoka; Tseeb, Vadim; Oyama, Kotaro; Ishiwata, Shin'ichi.

In: Biophysical Journal, Vol. 92, No. 6, 03.2007.

Research output: Contribution to journalArticle

Suzuki, Madoka ; Tseeb, Vadim ; Oyama, Kotaro ; Ishiwata, Shin'ichi. / Microscopic detection of thermogenesis in a single HeLa cell. In: Biophysical Journal. 2007 ; Vol. 92, No. 6.
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