Microscopic velocimetry with a scaled-up model for evaluating a flow field over cultured endothelial cells

Shuichiro Fukushima, Takaaki Deguchi, Makoto Kaibara, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A microscopic velocimetry technique for evaluating the flow field over cultured endothelial cells was developed. Flow around a cell model scaled up by a factor of 100 was visualized by using an optical microscope and was quantified by using particle-tracking velocimetry. Wall shear stress on the model surface was determined from a two-dimensional velocity field interpolated from measured velocity vectors. Accuracy of the velocimetry was verified by measuring the flow over a sinusoidal cell model that had a wall shear stress profile analytically determined with linear perturbation theory. Comparison of the experimental results with the analytical solution revealed that the total error of the measured wall shear stress was 6 percent.

Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalJournal of Biomechanical Engineering
Volume124
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

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Rheology
Endothelial cells
Velocity measurement
Shear stress
Cultured Cells
Flow fields
Endothelial Cells
Microscopes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics

Cite this

Microscopic velocimetry with a scaled-up model for evaluating a flow field over cultured endothelial cells. / Fukushima, Shuichiro; Deguchi, Takaaki; Kaibara, Makoto; Oka, Kotaro; Tanishita, Kazuo.

In: Journal of Biomechanical Engineering, Vol. 124, No. 2, 2002, p. 176-179.

Research output: Contribution to journalArticle

Fukushima, Shuichiro ; Deguchi, Takaaki ; Kaibara, Makoto ; Oka, Kotaro ; Tanishita, Kazuo. / Microscopic velocimetry with a scaled-up model for evaluating a flow field over cultured endothelial cells. In: Journal of Biomechanical Engineering. 2002 ; Vol. 124, No. 2. pp. 176-179.
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