Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale

Lan Zhang, Yuanhui Song, Atsushi Hosoi, Yasuyuki Morita, Yang Ju

Research output: Contribution to journalArticle

Abstract

In this paper, we report a non-invasive and non-destructive probing method for analyzing the MG63 osteoblast-like cells. High frequency microwave atomic force microscope (M-AFM) can be used to measure the surface topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation AFM mode, the M-AFM probe tip can scan above the cell surface, maintaining a constant stand-off distance and the created lateral forces were small enough as not to sweep away or deform the fragile biomolecules. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized. The unique potentials of that M-AFM imaging biological substrates with no damaging manner and nanometer scale resolution, while the original structure and function of the biomolecules during the investigation are preserved, make this technique very attractive to biologists.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalMicrosystem Technologies
Volume22
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1
Externally publishedYes

Fingerprint

osteoblasts
Osteoblasts
Microscopes
microscopes
Microwaves
Biomolecules
microwaves
cells
Microwave frequencies
Frequency modulation
Surface topography
organelles
Electric properties
frequency modulation
Proteins
Scanning
Imaging techniques
topography
electrical properties
atomic force microscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Hardware and Architecture
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Microwave atomic force microscope : MG63 osteoblast-like cells analysis on nanometer scale. / Zhang, Lan; Song, Yuanhui; Hosoi, Atsushi; Morita, Yasuyuki; Ju, Yang.

In: Microsystem Technologies, Vol. 22, No. 3, 01.03.2016, p. 603-608.

Research output: Contribution to journalArticle

Zhang, Lan ; Song, Yuanhui ; Hosoi, Atsushi ; Morita, Yasuyuki ; Ju, Yang. / Microwave atomic force microscope : MG63 osteoblast-like cells analysis on nanometer scale. In: Microsystem Technologies. 2016 ; Vol. 22, No. 3. pp. 603-608.
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