Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous

Toshio Ohshima, Edward C. Gilmore, Glenn Longenecker, David M. Jacobowitz, Roscoe O. Brady, Karl Herrup, Ashok B. Kulkarni

Research output: Contribution to journalArticle

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Abstract

Cyclin-dependent kinase 5 (Cdk5) is a member of the family of cell cycle-related kinases. Previous neuropathological analysis of cdk5(-/-) mice showed significant changes in CNS development in regions from cerebral cortex to brainstem. Among the defects in these animals, a disruption of the normal pattern of cell migrations in cerebellum was particularly apparent, including a pronounced abnormality in the location of cerebellar Purkinje cells. Complete analysis of this brain region is hampered in the mutant because most of cerebellar morphogenesis occurs after birth and the cdk5(-/-) mice die in the perinatal period. To overcome this disadvantage, we have generated chimeric mice by injection of cdk5(-/-) embryonic stem cells into host blastocysts. Analysis of the cerebellum from the resulting cdk5(-/-) mutually implies cdk5(+/+) chimeric mice shows that the abnormal location of the mutant Purkinje cells is a cell-autonomous defect. In addition, significant numbers of granule cells remain located in the molecular layer, suggesting a failure to complete migration from the external to the internal granule cell layer. In contrast to the Purkinje and granule cell populations, all three of the deep cerebellar nuclear cell groupings form correctly and are composed of cells of both mutant and wild-type genotypes. Despite similarities of the cdk5(-/-) phenotype to that reported in reeler and mdab-1(-/-) (scrambler/yotari) mutant brains, reelin and disabled-1 mRNA were found to be normal in cdk5(-/-) brain. Together, the data further support the hypothesis that Cdk5 activity is required for specific components of neuronal migration that are differentially required by different neuronal cell types and by even a single neuronal cell type at different developmental stages.

Original languageEnglish
Pages (from-to)6017-6026
Number of pages10
JournalJournal of Neuroscience
Volume19
Issue number14
Publication statusPublished - 1999 Jul 15
Externally publishedYes

Fingerprint

Cerebellum
Neurons
Purkinje Cells
Cyclin-Dependent Kinase 5
Brain
Blastocyst
Embryonic Stem Cells
Morphogenesis
Cerebral Cortex
Brain Stem
Cell Movement
Cell Cycle
Phosphotransferases
Cell Count
Genotype
Parturition
Phenotype
Messenger RNA
Injections
Population

Keywords

  • cdk5
  • Cell autonomous
  • Cerebellar development
  • Granule cell
  • Neuronal migration
  • Purkinje cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ohshima, T., Gilmore, E. C., Longenecker, G., Jacobowitz, D. M., Brady, R. O., Herrup, K., & Kulkarni, A. B. (1999). Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous. Journal of Neuroscience, 19(14), 6017-6026.

Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous. / Ohshima, Toshio; Gilmore, Edward C.; Longenecker, Glenn; Jacobowitz, David M.; Brady, Roscoe O.; Herrup, Karl; Kulkarni, Ashok B.

In: Journal of Neuroscience, Vol. 19, No. 14, 15.07.1999, p. 6017-6026.

Research output: Contribution to journalArticle

Ohshima, T, Gilmore, EC, Longenecker, G, Jacobowitz, DM, Brady, RO, Herrup, K & Kulkarni, AB 1999, 'Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous', Journal of Neuroscience, vol. 19, no. 14, pp. 6017-6026.
Ohshima T, Gilmore EC, Longenecker G, Jacobowitz DM, Brady RO, Herrup K et al. Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous. Journal of Neuroscience. 1999 Jul 15;19(14):6017-6026.
Ohshima, Toshio ; Gilmore, Edward C. ; Longenecker, Glenn ; Jacobowitz, David M. ; Brady, Roscoe O. ; Herrup, Karl ; Kulkarni, Ashok B. / Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 14. pp. 6017-6026.
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