MiR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes

Keita Tsujimura, Koichiro Irie, Hideyuki Nakashima, Yoshihiro Egashira, Yoichiro Fukao, Masayuki Fujiwara, Masayuki Itoh, Masahiro Uesaka, Takuya Imamura, Yasukazu Nakahata, Yui Yamashita, Takaya Abe, Shigeo Takamori, Kinichi Nakashima

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Rett syndrome (RTT) is a neurodevelopmental disorder caused by MECP2 mutations. Although emerging evidence suggests that MeCP2 deficiency is associated with dysregulation of mechanistic target of rapamycin (mTOR), which functions as a hub for various signaling pathways, the mechanism underlying this association and the molecular pathophysiology of RTT remain elusive. We show here that MeCP2 promotes the posttranscriptional processing of particular microRNAs (miRNAs) as a component of the microprocessor Drosha complex. Among the MeCP2-regulated miRNAs, we found that miR-199a positively controls mTOR signaling by targeting inhibitors for mTOR signaling. miR-199a and its targets have opposite effects on mTOR activity, ameliorating and inducing RTT neuronal phenotypes, respectively. Furthermore, genetic deletion of miR-199a-2 led to a reduction of mTOR activity in the brain and recapitulated numerous RTT phenotypes in mice. Together, these findings establish miR-199a as a critical downstream target of MeCP2 in RTT pathogenesis by linking MeCP2 with mTOR signaling.

Original languageEnglish
Pages (from-to)1887-1901
Number of pages15
JournalCell Reports
Volume12
Issue number11
DOIs
Publication statusPublished - 2015 Sep 22
Externally publishedYes

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Rett Syndrome
Sirolimus
Phenotype
MicroRNAs
Microcomputers
Microprocessor chips
Brain
Association reactions
Mutation
Processing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tsujimura, K., Irie, K., Nakashima, H., Egashira, Y., Fukao, Y., Fujiwara, M., ... Nakashima, K. (2015). MiR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes. Cell Reports, 12(11), 1887-1901. https://doi.org/10.1016/j.celrep.2015.08.028

MiR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes. / Tsujimura, Keita; Irie, Koichiro; Nakashima, Hideyuki; Egashira, Yoshihiro; Fukao, Yoichiro; Fujiwara, Masayuki; Itoh, Masayuki; Uesaka, Masahiro; Imamura, Takuya; Nakahata, Yasukazu; Yamashita, Yui; Abe, Takaya; Takamori, Shigeo; Nakashima, Kinichi.

In: Cell Reports, Vol. 12, No. 11, 22.09.2015, p. 1887-1901.

Research output: Contribution to journalArticle

Tsujimura, K, Irie, K, Nakashima, H, Egashira, Y, Fukao, Y, Fujiwara, M, Itoh, M, Uesaka, M, Imamura, T, Nakahata, Y, Yamashita, Y, Abe, T, Takamori, S & Nakashima, K 2015, 'MiR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes', Cell Reports, vol. 12, no. 11, pp. 1887-1901. https://doi.org/10.1016/j.celrep.2015.08.028
Tsujimura, Keita ; Irie, Koichiro ; Nakashima, Hideyuki ; Egashira, Yoshihiro ; Fukao, Yoichiro ; Fujiwara, Masayuki ; Itoh, Masayuki ; Uesaka, Masahiro ; Imamura, Takuya ; Nakahata, Yasukazu ; Yamashita, Yui ; Abe, Takaya ; Takamori, Shigeo ; Nakashima, Kinichi. / MiR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes. In: Cell Reports. 2015 ; Vol. 12, No. 11. pp. 1887-1901.
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