MiRNA profiling of bilateral rat hippocampal CA3 by deep sequencing

Yoshiaki Shinohara, Kazuko Yahagi, Mitsuoki Kawano, Hiromi Nishiyori, Chika Kawazu, Naoko Suzuki, Ri ichiroh Manabe, Hajime Hirase

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) have been demonstrated to be potent post-trascriptional modulators of protein expression. miRNA expression was profiled in the left and right dorsal hippocampal CA3 of mature rats by high-throughput deep sequencing. Among the sequenced and cross-mapped small RNAs, 88% belonged to the miRNAs annotated in the miRBase 15 database. Nearly half of the small RNAs belonged to the let-7 family miRNA. Seven percent of the sequenced small RNAs were not annotated in miRBase 15. Bioinformatic analysis of the unannotated small RNA sequences suggested seventeen novel miRNA candidates with relatively high expression levels (>100 tags per million). The left:right expression ratios were similar for all highly expressed miRNAs with less than 10% differences. These results provide a basic idea of the relative expression strengths of known and unknown miRNAs in the dorsal hippocampal CA3.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume409
Issue number2
DOIs
Publication statusPublished - 2011 Jun 3
Externally publishedYes

Keywords

  • Deep sequencing
  • Hippocampus
  • Left-right asymmetry
  • Let-7

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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