Mitochondrial DNA sequence variation within and between tuna Thunnus species and its application to species identification

Haruko Takeyama, S. Chow, H. Tsuzuki, Tadashi Matsunaga

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Restriction analysis detected two types of bigeye tuna (α and β); the α type was in the majority in the Atlantic but nearly absent in the Indo-Pacific. The α type shared a larger number of restriction sites with other species than the conspecific β type, but bigeye-specific nucleotide substitutions with a novel diagnostic restriction profile were found. Although the nucleotide sequence difference between Atlantic and Pacific sub-species of the northern bluefin tuna was nearly the largest among species, individuals possessing the Atlantic type of mtDNA were found at very low frequency in the Pacific and vice versa. Previous RFLP markers were found to be diagnostic for the other five species (albacore, blackfin, longtail, southern bluefin and yellowfin tunas). Genetic information is provided to discriminate all Thunnus species regardless of their origin and to identify the ocean of capture in the northern bluefin and bigeye tunas.

Original languageEnglish
Pages (from-to)1646-1657
Number of pages12
JournalJournal of Fish Biology
Volume58
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Thunnus
Thunnus obesus
Thunnus thynnus
tuna
mitochondrial DNA
Thunnus alalunga
nucleotide sequences
Thunnus albacares
restriction fragment length polymorphism
oceans
nucleotides
subspecies
substitution
species identification
sequence diversity
ocean
Thunnus maccoyii

Keywords

  • mtDNA sequence
  • RFLP
  • Species identification
  • Tuna

ASJC Scopus subject areas

  • Aquatic Science

Cite this

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title = "Mitochondrial DNA sequence variation within and between tuna Thunnus species and its application to species identification",
abstract = "Restriction analysis detected two types of bigeye tuna (α and β); the α type was in the majority in the Atlantic but nearly absent in the Indo-Pacific. The α type shared a larger number of restriction sites with other species than the conspecific β type, but bigeye-specific nucleotide substitutions with a novel diagnostic restriction profile were found. Although the nucleotide sequence difference between Atlantic and Pacific sub-species of the northern bluefin tuna was nearly the largest among species, individuals possessing the Atlantic type of mtDNA were found at very low frequency in the Pacific and vice versa. Previous RFLP markers were found to be diagnostic for the other five species (albacore, blackfin, longtail, southern bluefin and yellowfin tunas). Genetic information is provided to discriminate all Thunnus species regardless of their origin and to identify the ocean of capture in the northern bluefin and bigeye tunas.",
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T1 - Mitochondrial DNA sequence variation within and between tuna Thunnus species and its application to species identification

AU - Takeyama, Haruko

AU - Chow, S.

AU - Tsuzuki, H.

AU - Matsunaga, Tadashi

PY - 2001

Y1 - 2001

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