Mitochondrial methionyl-tRNA transformylase from bovine liver.

N. Takeuchi, M. Kawakami, T. Ueda, L. L. Spremulli, K. Watanabe

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Abstract

Substrate specificities of mammalian mitochondrial methionyl-tRNA transformylase (MTFmt) toward tRNA substrates were characterized in vitro. The MTFmt is able to formylate E. coli initiator methionyl-tRNA (Met-tRNA(fMet)) as efficiently as mammalian mitochondrial methionyl-tRNA. Furthermore, E. coli elongator methionyl-tRNA (Met-tRNA(mMet)) also serves as a substrate for mt MTF, whereas E. coli MTF rigorously excludes E. coli Met-tRNA(mMet) from formylation reaction. Thus, mammal mt MTF is suggested to have recognition mechanism different from E. coli MTF. To pursue the relationship between protein structure and unexpected substrate specificity of mammalian MTFmt, the nucleotide sequence of MTFmt gene was determined and its amino acids sequence was compared to other MTFs of prokaryotic origin.

Original languageEnglish
Pages (from-to)195-196
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997

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ASJC Scopus subject areas

  • Medicine(all)

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Takeuchi, N., Kawakami, M., Ueda, T., Spremulli, L. L., & Watanabe, K. (1997). Mitochondrial methionyl-tRNA transformylase from bovine liver. Nucleic acids symposium series, (37), 195-196.