Mitochondrial methionyl-tRNA transformylase from bovine liver.

N. Takeuchi, M. Kawakami, Takuya Ueda, L. L. Spremulli, K. Watanabe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Substrate specificities of mammalian mitochondrial methionyl-tRNA transformylase (MTFmt) toward tRNA substrates were characterized in vitro. The MTFmt is able to formylate E. coli initiator methionyl-tRNA (Met-tRNA(fMet)) as efficiently as mammalian mitochondrial methionyl-tRNA. Furthermore, E. coli elongator methionyl-tRNA (Met-tRNA(mMet)) also serves as a substrate for mt MTF, whereas E. coli MTF rigorously excludes E. coli Met-tRNA(mMet) from formylation reaction. Thus, mammal mt MTF is suggested to have recognition mechanism different from E. coli MTF. To pursue the relationship between protein structure and unexpected substrate specificity of mammalian MTFmt, the nucleotide sequence of MTFmt gene was determined and its amino acids sequence was compared to other MTFs of prokaryotic origin.

Original languageEnglish
Pages (from-to)195-196
Number of pages2
JournalNucleic acids symposium series
Issue number37
Publication statusPublished - 1997 Jan 1
Externally publishedYes

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methionyl-tRNA formyltransferase
RNA, Transfer, Met
Transfer RNA
Escherichia coli
Liver
Substrate Specificity
Mammals
Amino Acid Sequence

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Takeuchi, N., Kawakami, M., Ueda, T., Spremulli, L. L., & Watanabe, K. (1997). Mitochondrial methionyl-tRNA transformylase from bovine liver. Nucleic acids symposium series, (37), 195-196.

Mitochondrial methionyl-tRNA transformylase from bovine liver. / Takeuchi, N.; Kawakami, M.; Ueda, Takuya; Spremulli, L. L.; Watanabe, K.

In: Nucleic acids symposium series, No. 37, 01.01.1997, p. 195-196.

Research output: Contribution to journalArticle

Takeuchi, N, Kawakami, M, Ueda, T, Spremulli, LL & Watanabe, K 1997, 'Mitochondrial methionyl-tRNA transformylase from bovine liver.', Nucleic acids symposium series, no. 37, pp. 195-196.
Takeuchi N, Kawakami M, Ueda T, Spremulli LL, Watanabe K. Mitochondrial methionyl-tRNA transformylase from bovine liver. Nucleic acids symposium series. 1997 Jan 1;(37):195-196.
Takeuchi, N. ; Kawakami, M. ; Ueda, Takuya ; Spremulli, L. L. ; Watanabe, K. / Mitochondrial methionyl-tRNA transformylase from bovine liver. In: Nucleic acids symposium series. 1997 ; No. 37. pp. 195-196.
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