Modification of Hb-vesicles with oligosaccharide chains

H. Sakai, M. Takisada, J. E. Chung, Shinji Takeoka, E. Tsuchida

    Research output: Contribution to journalArticle

    Abstract

    Synthetic glycolipids were introduced into lipid membranes of Hb-vesicles in order to modify the surface of the vesicles with oligosaccharide chains. The resulting suspension of the Hb-vesicles showed good rheological properties and high structural stability. Intervesicular aggregation induced by the addition of Ca2+ (5 mM) or dextran (Mw. 40,000) was suppressed due to the excluded volume effect of the oligosaccharide chains which inhibited the access of the surrounding vesicles. After freeze-thawing of the Hb-vesicles with oligosaccharide chains, no diameter change was observed, and leakage of Hb was also suppressed. No change in oxygen binding properties indicates that small molecular or ions as allosteric effectors were not leaked from the vesicles. Therefore, the Hb-vesicles can be stored in a frozen state by the surface modification of the vesicles with the oligosaccharide chains.

    Original languageEnglish
    Pages (from-to)864-867
    Number of pages4
    JournalJapanese Journal of Artificial Organs
    Volume23
    Issue number3
    Publication statusPublished - 1994

    Fingerprint

    Oligosaccharides
    Glycolipids
    Membrane Lipids
    Dextrans
    Suspensions
    Ions
    Oxygen

    Keywords

    • freeze-thawing
    • Hb-vesicles
    • intervesicular aggregation
    • oligosaccharide chains
    • synthetic glycolipid

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Sakai, H., Takisada, M., Chung, J. E., Takeoka, S., & Tsuchida, E. (1994). Modification of Hb-vesicles with oligosaccharide chains. Japanese Journal of Artificial Organs, 23(3), 864-867.

    Modification of Hb-vesicles with oligosaccharide chains. / Sakai, H.; Takisada, M.; Chung, J. E.; Takeoka, Shinji; Tsuchida, E.

    In: Japanese Journal of Artificial Organs, Vol. 23, No. 3, 1994, p. 864-867.

    Research output: Contribution to journalArticle

    Sakai, H, Takisada, M, Chung, JE, Takeoka, S & Tsuchida, E 1994, 'Modification of Hb-vesicles with oligosaccharide chains', Japanese Journal of Artificial Organs, vol. 23, no. 3, pp. 864-867.
    Sakai, H. ; Takisada, M. ; Chung, J. E. ; Takeoka, Shinji ; Tsuchida, E. / Modification of Hb-vesicles with oligosaccharide chains. In: Japanese Journal of Artificial Organs. 1994 ; Vol. 23, No. 3. pp. 864-867.
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