Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II

Yoshikazu Suzuki, Reiko Ohkura, Seiryo Sugiura, Ryohei Yasuda, Kazuhiko Kinoshita, Masaru Tanokura, Kazuo Sutoh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cysteine residue called SH2 in the skeletal myosin heavy chain is conserved among various species. Cys 678 in Dictyostelium myosin II is equivalent to SH2 in skeletal myosin. Using the Dictyostelium myosin II heavy chain gene, SH2 was mutated to Gly, Ala, Ser, or Thr. These mutant myosins were expressed in Dictyostelium myosin-null cells. To investigate how these mutations affect the motor functions of myosin, we examined the phenotypes of the transformed cells. We also purified the mutant myosins, and characterized them by measuring the actin-activated MgATPase activity, sliding velocity of actin filaments and force level. All of these mutant myosins complemented the myosin-specific defects of the myosin-null cells. Consistent with these observed phenotypes, all of the purified mutant myosins retained similar actin-activated MgATPase activities and force levels to those of the wild-type myosin (WT). However, the sliding velocities of actin filaments were significantly different (WT ≤ Ser > Ala >> Thr > Gly). In particular, the Gly and Thr mutants exhibited a striking decrease in velocity, while the Ser mutant exhibited velocity comparable to that of the wild-type myosin. Thus, mutations of SH2 resulted in uncoupling of ATP hydrolysis and the sliding.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume234
Issue number3
DOIs
Publication statusPublished - 1997 May 29
Externally publishedYes

Fingerprint

Myosin Type II
Dictyostelium
Myosins
Actin Cytoskeleton
Cysteine
Actins
Modulation
Mutation
Null Lymphocytes
Myosin Heavy Chains
Phenotype
Hydrolysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II. / Suzuki, Yoshikazu; Ohkura, Reiko; Sugiura, Seiryo; Yasuda, Ryohei; Kinoshita, Kazuhiko; Tanokura, Masaru; Sutoh, Kazuo.

In: Biochemical and Biophysical Research Communications, Vol. 234, No. 3, 29.05.1997, p. 701-706.

Research output: Contribution to journalArticle

Suzuki, Y, Ohkura, R, Sugiura, S, Yasuda, R, Kinoshita, K, Tanokura, M & Sutoh, K 1997, 'Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II', Biochemical and Biophysical Research Communications, vol. 234, no. 3, pp. 701-706. https://doi.org/10.1006/bbrc.1997.6671
Suzuki, Yoshikazu ; Ohkura, Reiko ; Sugiura, Seiryo ; Yasuda, Ryohei ; Kinoshita, Kazuhiko ; Tanokura, Masaru ; Sutoh, Kazuo. / Modulation of actin filament sliding by mutations of the SH2 cysteine in Dictyostelium myosin II. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 234, No. 3. pp. 701-706.
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