Modulation of cell mutability by serum factors from volunteers who experienced head-down-tilt bed rest.

Shigeru Sugaya, Kazuko Kita, Hideoki Fukuoka, Nobuo Suzuki

Research output: Contribution to journalArticle

Abstract

Head-down-tilt bed rest (BR) is utilized to mimic a microgravity environment. In this study, we examined whether the modulating activity of serum factors on cell mutability was changed in seventeen volunteers subjected to BR. For comparison, serum was comparatively obtained from seven volunteers who experienced the parabolic flight (PF). Cultured human cells were treated with serum and then irradiated with UV or X-ray. Mutagenicity was estimated by identification of K-ras codon 12 base substitution mutation, following PCR and differential dot-blot hybridization. Modulating activity was detected in one sample from each of post-BR and post-PF serum.

Original languageEnglish
Pages (from-to)167-168
Number of pages2
JournalBiological sciences in space = Uchū seibutsu kagaku
Volume18
Issue number3
Publication statusPublished - 2004
Externally publishedYes

    Fingerprint

Cite this