Modulation of eosinophil chemotactic activities to leukotriene B4 by n-3 polyunsaturated fatty acids

S. Kikuchi, T. Sakamoto, C. Ishikawa, Kazunaga Yazawa, S. Torii

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Eosinophil accumulation induced by leukotriene B4 appears to be involved in the pathogenesis of allergic diseases. We evaluated the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on chemotaxis to leukotriene B4 in guinea pig peritoneal eosinophils. Guinea pigs that were sensitized to polymyxin B were administered an intraperitoneal injection of polymyxin B (1 mg/animal) alone or combined with DHA (15 or 50 mg/kg, i.p.), EPA (50 or 100 mg/kg, i.p.), or with linoleic acid (LA) (100 mg/kg, i.p.). Forty hours later, eosinophils were obtained from the intraperitoneal ravage fluid and purified. The chemotactic and chemokinetic responses of eosinophils to leukotriene 3, were measured using a 96-well microchemotaxis chamber. DHA significantly decreased the chemotactic and chemokinetic responses of eosinophils in a dose-dependent fashion. A higher dose of EPA also significantly inhibited both of those responses, whereas LA had no effect. Our results suggested a possible mechanism for the improvement of allergic diseases by dietary supplementation with n-3 PUFA.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume58
Issue number3
DOIs
Publication statusPublished - 1998 Mar
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

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