Modulation of the mechano-chemical properties of myosin V by drebrin-E

Hiroaki Kubota, Ryoki Ishikawa, Takashi Ohki, Junji Ishizuka, Sergey Mikhailenko, Shin'ichi Ishiwata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The regulation of actin filament networks by various proteins has essential roles in the growth cone dynamics. In this study we focused on the actin-myosin interaction which has been suggested to be an important player in the neurite extension. We examined in vitro how the decoration of actin filaments with a side-binding protein, drebrin-E, affects the motile properties of an intracellular transporter myosin V. Single myosin V molecules landed on the drebrin-E-decorated actin filaments with a lower frequency and ran over shorter distances; however, their velocities were normal. Furthermore, the analysis of the movement of myosin V molecules in the optical trap revealed that the decoration of actin filaments with drebrin-E markedly increased the load-sensitivity of the myosin V stepping. These results are attributable to the delay in the attachment of the motor's leading head (ADP·Pi state) to actin, induced by the competitive binding of drebrin-E to actin, whereas the rate of ADP release from the trailing head (the rate-limiting step in the ATPase cycle of myosin V) is unaffected. Our study indicates that, in addition to the regulation of binding affinity of myosin V, drebrin-E also modulates the chemo-mechanical coupling in the motile myosin V molecules, presumably affecting the movement of the growth cone.

Original languageEnglish
Pages (from-to)643-648
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume400
Issue number4
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Myosin Type V
Chemical properties
Actins
Modulation
Actin Cytoskeleton
Growth Cones
Molecules
Cones
Head
Optical Tweezers
Competitive Binding
Neurites
Myosins
drebrin E
Adenosine Diphosphate
Adenosine Triphosphatases
Carrier Proteins

Keywords

  • Actin
  • Drebrin
  • Myosin V
  • Optical tweezers
  • Single-molecule

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Modulation of the mechano-chemical properties of myosin V by drebrin-E. / Kubota, Hiroaki; Ishikawa, Ryoki; Ohki, Takashi; Ishizuka, Junji; Mikhailenko, Sergey; Ishiwata, Shin'ichi.

In: Biochemical and Biophysical Research Communications, Vol. 400, No. 4, 01.10.2010, p. 643-648.

Research output: Contribution to journalArticle

Kubota, Hiroaki ; Ishikawa, Ryoki ; Ohki, Takashi ; Ishizuka, Junji ; Mikhailenko, Sergey ; Ishiwata, Shin'ichi. / Modulation of the mechano-chemical properties of myosin V by drebrin-E. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 400, No. 4. pp. 643-648.
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