Modulation of viability of live cells by focused ion-beam exposure

Takahiro Shinada, Takayuki Akimoto, Yanwei Zhu, Hisa Goke, Iwao Ohdomari

Research output: Contribution to journalLetter

2 Citations (Scopus)

Abstract

Introduction of membrane-impermeant substances into living cells is the key method to understand contemporary cellular processes by investigating cellular responses and phenotypes. Here, we performed gold ion beam exposure into live cells by using the focused ion beam implantation method, which was originally developed to precisely control semiconductor device performances. We evaluated the viability of the gold-irradiated cells by measuring the concentration of adenosine triphosphate (ATP), which is an intracellular energy source produced in the mitochondrial membrane. The viability of the irradiated cells was found to be 20% higher than that of the unirradiated control cells. The atoms might promote the energy generating processes within the mitochondrion. Our results suggest that the viability of living cells can be modulated by accurately controlling the dopant atom numbers. Our technique may be considered as a potential tool in life and medical sciences to quantitatively elucidate the dose-dependent effects of dopants.

Original languageEnglish
Pages (from-to)222-225
Number of pages4
JournalBiotechnology and Bioengineering
Volume108
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Fingerprint

Focused ion beams
Cell Survival
Cells
Modulation
Ions
Gold
Doping (additives)
Membranes
Atoms
Semiconductors
Mitochondria
Biological Science Disciplines
Mitochondrial Membranes
Semiconductor devices
Ion beams
Adenosine Triphosphate
Phenotype
Equipment and Supplies

Keywords

  • Cell viability
  • Focused ion beam
  • Ion beam exposure

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Modulation of viability of live cells by focused ion-beam exposure. / Shinada, Takahiro; Akimoto, Takayuki; Zhu, Yanwei; Goke, Hisa; Ohdomari, Iwao.

In: Biotechnology and Bioengineering, Vol. 108, No. 1, 01.2011, p. 222-225.

Research output: Contribution to journalLetter

Shinada, Takahiro ; Akimoto, Takayuki ; Zhu, Yanwei ; Goke, Hisa ; Ohdomari, Iwao. / Modulation of viability of live cells by focused ion-beam exposure. In: Biotechnology and Bioengineering. 2011 ; Vol. 108, No. 1. pp. 222-225.
@article{ea43b9e78e994d17b77735b09608a442,
title = "Modulation of viability of live cells by focused ion-beam exposure",
abstract = "Introduction of membrane-impermeant substances into living cells is the key method to understand contemporary cellular processes by investigating cellular responses and phenotypes. Here, we performed gold ion beam exposure into live cells by using the focused ion beam implantation method, which was originally developed to precisely control semiconductor device performances. We evaluated the viability of the gold-irradiated cells by measuring the concentration of adenosine triphosphate (ATP), which is an intracellular energy source produced in the mitochondrial membrane. The viability of the irradiated cells was found to be 20{\%} higher than that of the unirradiated control cells. The atoms might promote the energy generating processes within the mitochondrion. Our results suggest that the viability of living cells can be modulated by accurately controlling the dopant atom numbers. Our technique may be considered as a potential tool in life and medical sciences to quantitatively elucidate the dose-dependent effects of dopants.",
keywords = "Cell viability, Focused ion beam, Ion beam exposure",
author = "Takahiro Shinada and Takayuki Akimoto and Yanwei Zhu and Hisa Goke and Iwao Ohdomari",
year = "2011",
month = "1",
doi = "10.1002/bit.22917",
language = "English",
volume = "108",
pages = "222--225",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Modulation of viability of live cells by focused ion-beam exposure

AU - Shinada, Takahiro

AU - Akimoto, Takayuki

AU - Zhu, Yanwei

AU - Goke, Hisa

AU - Ohdomari, Iwao

PY - 2011/1

Y1 - 2011/1

N2 - Introduction of membrane-impermeant substances into living cells is the key method to understand contemporary cellular processes by investigating cellular responses and phenotypes. Here, we performed gold ion beam exposure into live cells by using the focused ion beam implantation method, which was originally developed to precisely control semiconductor device performances. We evaluated the viability of the gold-irradiated cells by measuring the concentration of adenosine triphosphate (ATP), which is an intracellular energy source produced in the mitochondrial membrane. The viability of the irradiated cells was found to be 20% higher than that of the unirradiated control cells. The atoms might promote the energy generating processes within the mitochondrion. Our results suggest that the viability of living cells can be modulated by accurately controlling the dopant atom numbers. Our technique may be considered as a potential tool in life and medical sciences to quantitatively elucidate the dose-dependent effects of dopants.

AB - Introduction of membrane-impermeant substances into living cells is the key method to understand contemporary cellular processes by investigating cellular responses and phenotypes. Here, we performed gold ion beam exposure into live cells by using the focused ion beam implantation method, which was originally developed to precisely control semiconductor device performances. We evaluated the viability of the gold-irradiated cells by measuring the concentration of adenosine triphosphate (ATP), which is an intracellular energy source produced in the mitochondrial membrane. The viability of the irradiated cells was found to be 20% higher than that of the unirradiated control cells. The atoms might promote the energy generating processes within the mitochondrion. Our results suggest that the viability of living cells can be modulated by accurately controlling the dopant atom numbers. Our technique may be considered as a potential tool in life and medical sciences to quantitatively elucidate the dose-dependent effects of dopants.

KW - Cell viability

KW - Focused ion beam

KW - Ion beam exposure

UR - http://www.scopus.com/inward/record.url?scp=78649419381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649419381&partnerID=8YFLogxK

U2 - 10.1002/bit.22917

DO - 10.1002/bit.22917

M3 - Letter

VL - 108

SP - 222

EP - 225

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 1

ER -