Molecular analysis of halophilic bacterial community for high-rate denitrification of saline industrial wastewater

Sachiko Yoshie, Hiroshi Makino, Hidenobu Hirosawa, Kosuke Shirotani, Satoshi Tsuneda, Akira Hirata

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    A denitrification system for saline wastewater utilizing halophilic denitrifying bacteria has not been developed so far. In this study, denitrification performance and microbial community under various saline conditions were investigated using denitrifying sludge acclimated under low-salinity condition for a few years as seed sludge. A continuous denitrification experiment showed that denitrification performance and microbial community at 10% salinity was higher than that at 1% salinity. The microbial community in the denitrification sludge that was acclimated under low salinity was monitored by terminal-restriction fragment length polymorphism (T-RFLP) analysis during acclimation to high-salinity condition. T-RFLP profiles and clone analysis based on 16S rRNA-encoding genes in the sludge of the denitrification system with 10% salinity indicated that the γ- Proteobacteria, particularly Halomonas spp., were predominant species, suggesting that these bacterial members were possibly responsible for a high denitrification activity under high-salinity conditions. Furthermore, the investigation of denitrification performance under various saline conditions revealed that 4-10% salinity results in the highest denitrification rate, indicating that this salinity was optimal for predominant bacterial species to exhibit denitrification activity. These results indicate the possibility that an appropriate denitrification system for saline wastewater can be designed using acclimated sludge with a halophilic community.

    Original languageEnglish
    Pages (from-to)182-189
    Number of pages8
    JournalApplied Microbiology and Biotechnology
    Volume72
    Issue number1
    DOIs
    Publication statusPublished - 2006 Aug

    Fingerprint

    Denitrification
    Waste Water
    Salinity
    Wastewater
    Sewage
    Polymorphism
    Restriction Fragment Length Polymorphisms
    Halomonas
    Proteobacteria
    Gene encoding
    Acclimatization
    rRNA Genes
    Seed
    Seeds
    Bacteria
    Clone Cells

    ASJC Scopus subject areas

    • Biotechnology
    • Microbiology
    • Bioengineering
    • Microbiology (medical)

    Cite this

    Molecular analysis of halophilic bacterial community for high-rate denitrification of saline industrial wastewater. / Yoshie, Sachiko; Makino, Hiroshi; Hirosawa, Hidenobu; Shirotani, Kosuke; Tsuneda, Satoshi; Hirata, Akira.

    In: Applied Microbiology and Biotechnology, Vol. 72, No. 1, 08.2006, p. 182-189.

    Research output: Contribution to journalArticle

    Yoshie, Sachiko ; Makino, Hiroshi ; Hirosawa, Hidenobu ; Shirotani, Kosuke ; Tsuneda, Satoshi ; Hirata, Akira. / Molecular analysis of halophilic bacterial community for high-rate denitrification of saline industrial wastewater. In: Applied Microbiology and Biotechnology. 2006 ; Vol. 72, No. 1. pp. 182-189.
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