Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process

T. Hoshino, T. Terahara, Satoshi Tsuneda, A. Hirata, Y. Inamori

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    Aims: The objective of this study is to determine the bacteria playing an important role in denitrification by monitoring the molecular dynamics accompanying the start of denitrification. Methods and Results: cDNA reverse-transcribed from 16S rRNA was amplified with fluorescent labelled primer for terminal restriction fragment length polymorphism (T-RFLP) analysis and an unlabelled primer for cloning analysis. The terminal restriction fragments (T-RFs) that increased in association with the start of denitrification were determined. These T-RFs were identified by in silico analysis of 16S rRNA sequences obtained from cloning. As a result, it was clearly observed that the bacteria belonging to the genera Hydrogenophaga and Acidovorax increased in number after the start of denitrification. Conclusions: It was demonstrated that T-RFLP analysis targeting 16S rRNA is appropriate for the daily monitoring of a bacterial community to control wastewater treatment processes. Combination of the results of T-RFLP analysis and 16S rRNA clone library indicated that the bacteria belonging to the genera Hydrogenophaga and Acidovorax play an important role in denitrification. Significance and Impact of the Study: The results of this study provide new insight to the 16S rRNA level of active denitrifying bacteria in wastewater treatment processes.

    Original languageEnglish
    Pages (from-to)1165-1175
    Number of pages11
    JournalJournal of Applied Microbiology
    Volume99
    Issue number5
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Denitrification
    Comamonadaceae
    Waste Water
    wastewater treatment
    denitrification
    ribosomal RNA
    Hydrogenophaga
    Acidovorax
    Restriction Fragment Length Polymorphisms
    Bacteria
    restriction fragment length polymorphism
    Population
    Organism Cloning
    molecular cloning
    bacteria
    denitrifying bacteria
    Therapeutics
    molecular dynamics
    monitoring
    Molecular Dynamics Simulation

    Keywords

    • Denitrifying bacteria
    • Monitoring
    • T-RFLP
    • Wastewater treatment process

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Applied Microbiology and Biotechnology
    • Biotechnology
    • Microbiology

    Cite this

    Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process. / Hoshino, T.; Terahara, T.; Tsuneda, Satoshi; Hirata, A.; Inamori, Y.

    In: Journal of Applied Microbiology, Vol. 99, No. 5, 2005, p. 1165-1175.

    Research output: Contribution to journalArticle

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