Molecular cloning and characterization of a cDNA encoding the retinal arylalkylamine N-acetyltransferase of the rainbow trout, Oncorhynchus mykiss

Kanta Mizusawa, Masayuki Iigo, Hiroaki Suetake, Yasutoshi Yoshiura, Koichiro Gen, Kiyoshi Kikuchi, Toshiyuki Okano, Yoshitaka Fukada, Katsumi Aida

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Melatonin synthesis in the retina as well as in the pineal gland exhibits daily variations with higher levels during the dark phase of light-dark cycles. To analyze the molecular mechanism of melatonin synthesis in the retina, we have cloned, sequenced and characterized a putative cDNA for arylalkylamine N-acetyltransferase (AANAT; EC 2.3.1.87), a rate-limiting enzyme in melatonin production, from the retina of the rainbow trout (Oncorhynchus mykiss). The trout AANAT cDNA (1,585 bp) contains an open reading frame encoding 240 amino acid protein (predicted molecular weight, 27,420) that is 51-65% identical to avian and mammalian AANAT. The trout retinal AANAT protein contains motifs A and B that are conserved among the N-acetyltransferase superfamily and eight potential phosphorylation sites. Southern blot analysis demonstrated that the protein is expressed by a single copy gene. A single AANAT transcript (1.6 kb) was detected in the retina but not in the liver by Northern blot analysis. The levels of AANAT mRNA in the retina exhibited day-night changes with 3.3-fold increase at night. These results indicate that in the rainbow trout retina, the activity of AANAT and thus melatonin synthesis are regulated at least in part at the transcriptional level.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalZoological Science
Volume15
Issue number3
Publication statusPublished - 1998 Jun
Externally publishedYes

Fingerprint

molecular cloning
Oncorhynchus mykiss
retina
melatonin
trout
synthesis
pineal body
acetyltransferases
proteins
aralkylamine N-acetyltransferase
Northern blotting
Southern blotting
open reading frames
phosphorylation
photoperiod
molecular weight
liver
amino acids
enzymes
genes

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Mizusawa, K., Iigo, M., Suetake, H., Yoshiura, Y., Gen, K., Kikuchi, K., ... Aida, K. (1998). Molecular cloning and characterization of a cDNA encoding the retinal arylalkylamine N-acetyltransferase of the rainbow trout, Oncorhynchus mykiss. Zoological Science, 15(3), 345-351.

Molecular cloning and characterization of a cDNA encoding the retinal arylalkylamine N-acetyltransferase of the rainbow trout, Oncorhynchus mykiss. / Mizusawa, Kanta; Iigo, Masayuki; Suetake, Hiroaki; Yoshiura, Yasutoshi; Gen, Koichiro; Kikuchi, Kiyoshi; Okano, Toshiyuki; Fukada, Yoshitaka; Aida, Katsumi.

In: Zoological Science, Vol. 15, No. 3, 06.1998, p. 345-351.

Research output: Contribution to journalArticle

Mizusawa, K, Iigo, M, Suetake, H, Yoshiura, Y, Gen, K, Kikuchi, K, Okano, T, Fukada, Y & Aida, K 1998, 'Molecular cloning and characterization of a cDNA encoding the retinal arylalkylamine N-acetyltransferase of the rainbow trout, Oncorhynchus mykiss', Zoological Science, vol. 15, no. 3, pp. 345-351.
Mizusawa, Kanta ; Iigo, Masayuki ; Suetake, Hiroaki ; Yoshiura, Yasutoshi ; Gen, Koichiro ; Kikuchi, Kiyoshi ; Okano, Toshiyuki ; Fukada, Yoshitaka ; Aida, Katsumi. / Molecular cloning and characterization of a cDNA encoding the retinal arylalkylamine N-acetyltransferase of the rainbow trout, Oncorhynchus mykiss. In: Zoological Science. 1998 ; Vol. 15, No. 3. pp. 345-351.
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