Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein D (PLP-D)

Ken Iwatsuki, Mikihiko Shinozaki, Naka Hattori, Kensuke Hirasawa, Shin Ichi Itagaki, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The rat placental PRL family consists of molecules structurally similar to PRL and GH, and to date, seven members have been identified. During investigation of pregnancy stage-specific placental factors by the differential display method, we obtained a complementary DNA (cDNA) fragment (199 bp) encoding a peptide homologous to PRL-like protein (PLP)-C. By using the 3' and 5' rapid amplification of cDNA ends method, a full-length cDNA was cloned and tentatively named PLP-D. The cDNA encoded a mature protein of 240 amino acids, including a 29-amino acid signal sequence. PLP-D contains one putative N-glycosylation site and six cysteine residues that are highly conserved in the placental PRL family. Sequence comparison between PLP-D and other members of the placental PRL family showed that PLP-D is highly homologous to PLP-C (80%) and decidual PRL-related protein (73%). Northern blot analysis revealed that PLP-D messenger RNA (mRNA) first appeared at day 14 of pregnancy, and that its expression increased until term. In situ hybridization analysis indicated that PLP-D mRNA was specifically expressed in spongiotrophoblast cells and trophoblast giant cells of the placental junctional zone. Differentiated Rcho-1 cells also expressed PLP-D mRNA, whereas undifferentiated Rcho-1 cells did not.

Original languageEnglish
Pages (from-to)3849-3855
Number of pages7
JournalEndocrinology
Volume137
Issue number9
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Molecular Cloning
Prolactin
Complementary DNA
Proteins
Protein C
Messenger RNA
Pregnancy
Trophoblasts
Giant Cells
Protein Sorting Signals
PRL-like protein D
Glycosylation
Northern Blotting
In Situ Hybridization
Cysteine
Amino Acid Sequence
Amino Acids
Peptides
DNA-like RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Iwatsuki, K., Shinozaki, M., Hattori, N., Hirasawa, K., Itagaki, S. I., Shiota, K., & Ogawa, T. (1996). Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein D (PLP-D). Endocrinology, 137(9), 3849-3855. https://doi.org/10.1210/en.137.9.3849

Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein D (PLP-D). / Iwatsuki, Ken; Shinozaki, Mikihiko; Hattori, Naka; Hirasawa, Kensuke; Itagaki, Shin Ichi; Shiota, Kunio; Ogawa, Tomoya.

In: Endocrinology, Vol. 137, No. 9, 1996, p. 3849-3855.

Research output: Contribution to journalArticle

Iwatsuki, K, Shinozaki, M, Hattori, N, Hirasawa, K, Itagaki, SI, Shiota, K & Ogawa, T 1996, 'Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein D (PLP-D)', Endocrinology, vol. 137, no. 9, pp. 3849-3855. https://doi.org/10.1210/en.137.9.3849
Iwatsuki, Ken ; Shinozaki, Mikihiko ; Hattori, Naka ; Hirasawa, Kensuke ; Itagaki, Shin Ichi ; Shiota, Kunio ; Ogawa, Tomoya. / Molecular cloning and characterization of a new member of the rat placental prolactin (PRL) family, PRL-like protein D (PLP-D). In: Endocrinology. 1996 ; Vol. 137, No. 9. pp. 3849-3855.
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