Molecular cloning and characterization of anti-Müllerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa

Maho Kodama, Mari Suda, Daiki Sakamoto, Takehiro Iwasaki, Yasuki Matsuo, Yoshinobu Uno, Yoichi Matsuda, Yoriko Nakamura, Shun Maekawa, Yoshinao Katsu, Masahisa Nakamura

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    9 Citations (Scopus)

    Abstract

    The role of anti-Müllerian hormone (AMH) during gonad development has been studied extensively in many species of mammal, bird, reptile, and fish but remains unresolved in amphibians. In male mammalian embryos, Sox9 activates AMH expression, which initiates regression of the Müllerian ducts. However, Sox9 (Sry-relatedHMGbox 9) is unlikely to initiateAMHin chicken, because AMH precedes Sox9 expression in this species. To clarify whether AMH is involved in testicular differentiation in amphibians, we cloned the full-length AMH cDNA from the Japanese wrinkled frog, Rana rugosa. The AMH gene, which appears to be autosomal, is exclusively expressed in the testis of adult frog among 8 different tissues examined; Sertoli cells are probably responsible for its expression. AMH expression was found in the undifferentiated gonad of both male and female tadpoles, increasing in the differentiating testis. Moreover, we observed consensus binding sites for Sox9 in the 5'-flanking region of the AMH gene. Sox9 stimulated statistically significant AMH expression in luciferase reporter assayswhencoexpressed in Xenopus kidney-derivedA6cells. However, Sox9 expression showed no sexual dimorphism when AMH expression was up-regulated in the developing testis. These results, taken together, suggest thatAMHis probably involved in testicular differentiation in R. rugosa, although an additional, perhaps tissue-specific, transcription factor may be required for the regulation of AMH transcription.

    Original languageEnglish
    Pages (from-to)1914-1923
    Number of pages10
    JournalEndocrinology
    Volume156
    Issue number5
    DOIs
    Publication statusPublished - 2015 May 1

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    Ranidae
    Molecular Cloning
    Anura
    Hormones
    Testis
    Gonads
    Amphibians
    Mammalian Embryo
    Reptiles
    Sertoli Cells
    5' Flanking Region
    Xenopus
    Luciferases
    Sex Characteristics
    Genes
    Birds
    Larva
    Mammals
    Chickens
    Fishes

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Kodama, M., Suda, M., Sakamoto, D., Iwasaki, T., Matsuo, Y., Uno, Y., ... Nakamura, M. (2015). Molecular cloning and characterization of anti-Müllerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa. Endocrinology, 156(5), 1914-1923. https://doi.org/10.1210/en.2013-2053

    Molecular cloning and characterization of anti-Müllerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa. / Kodama, Maho; Suda, Mari; Sakamoto, Daiki; Iwasaki, Takehiro; Matsuo, Yasuki; Uno, Yoshinobu; Matsuda, Yoichi; Nakamura, Yoriko; Maekawa, Shun; Katsu, Yoshinao; Nakamura, Masahisa.

    In: Endocrinology, Vol. 156, No. 5, 01.05.2015, p. 1914-1923.

    Research output: Contribution to journalArticle

    Kodama, M, Suda, M, Sakamoto, D, Iwasaki, T, Matsuo, Y, Uno, Y, Matsuda, Y, Nakamura, Y, Maekawa, S, Katsu, Y & Nakamura, M 2015, 'Molecular cloning and characterization of anti-Müllerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa', Endocrinology, vol. 156, no. 5, pp. 1914-1923. https://doi.org/10.1210/en.2013-2053
    Kodama, Maho ; Suda, Mari ; Sakamoto, Daiki ; Iwasaki, Takehiro ; Matsuo, Yasuki ; Uno, Yoshinobu ; Matsuda, Yoichi ; Nakamura, Yoriko ; Maekawa, Shun ; Katsu, Yoshinao ; Nakamura, Masahisa. / Molecular cloning and characterization of anti-Müllerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa. In: Endocrinology. 2015 ; Vol. 156, No. 5. pp. 1914-1923.
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    AU - Matsuo, Yasuki

    AU - Uno, Yoshinobu

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