Molecular cloning and chromosomal localization of the human thrombopoietin gene Yoshiaki Sohma a, Hiromichi Akahori a, Naohiko Seki b,c, Tada-aki Hori b, Kinya Ogami a

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Abstract

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by SI nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalFEBS Letters
Volume353
Issue number1
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Keywords

  • Chromosome mapping
  • Genomic DNA
  • Thrombopoietin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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