Molecular cloning and chromosomal localization of the human thrombopoietin gene Yoshiaki Sohma a, Hiromichi Akahori a, Naohiko Seki b,c, Tada-aki Hori b, Kinya Ogami a

Research output: Contribution to journalArticle

Abstract

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by SI nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalFEBS Letters
Volume353
Issue number1
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Thrombopoietin
Cloning
Molecular Cloning
Genes
Genomic Library
Transcription Initiation Site
COS Cells
Human Genome
Chromosomes
Biotin
Southern Blotting
Introns
In Situ Hybridization
Exons
Screening
Complementary DNA

Keywords

  • Chromosome mapping
  • Genomic DNA
  • Thrombopoietin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{800f3293d8794829b911094d1dcdae9a,
title = "Molecular cloning and chromosomal localization of the human thrombopoietin gene Yoshiaki Sohma a, Hiromichi Akahori a, Naohiko Seki b,c, Tada-aki Hori b, Kinya Ogami a",
abstract = "The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by SI nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.",
keywords = "Chromosome mapping, Genomic DNA, Thrombopoietin",
author = "Takashi Kato",
year = "1994",
language = "English",
volume = "353",
pages = "57--61",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Molecular cloning and chromosomal localization of the human thrombopoietin gene Yoshiaki Sohma a, Hiromichi Akahori a, Naohiko Seki b,c, Tada-aki Hori b, Kinya Ogami a

AU - Kato, Takashi

PY - 1994

Y1 - 1994

N2 - The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by SI nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

AB - The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by SI nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

KW - Chromosome mapping

KW - Genomic DNA

KW - Thrombopoietin

UR - http://www.scopus.com/inward/record.url?scp=28044468682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28044468682&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:28044468682

VL - 353

SP - 57

EP - 61

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -