Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma, Hiromichi Akahori, Naohiko Seki, Tada aki Hori, Kinya Ogami, Takashi Kato, Yoshihiro Shimada, Kazuo Kawamura, Hiroshi Miyazaki

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalFEBS Letters
Volume353
Issue number1
DOIs
Publication statusPublished - 1994 Oct 10
Externally publishedYes

Fingerprint

Thrombopoietin
Cloning
Molecular Cloning
Genes
Genomic Library
Transcription Initiation Site
COS Cells
Human Genome
Chromosomes
Biotin
Southern Blotting
Introns
In Situ Hybridization
Exons
Screening
Complementary DNA

Keywords

  • Chromosome mapping
  • Genomic DNA
  • Thrombopoietin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sohma, Y., Akahori, H., Seki, N., Hori, T. A., Ogami, K., Kato, T., ... Miyazaki, H. (1994). Molecular cloning and chromosomal localization of the human thrombopoietin gene. FEBS Letters, 353(1), 57-61. https://doi.org/10.1016/0014-5793(94)01008-0

Molecular cloning and chromosomal localization of the human thrombopoietin gene. / Sohma, Yoshiaki; Akahori, Hiromichi; Seki, Naohiko; Hori, Tada aki; Ogami, Kinya; Kato, Takashi; Shimada, Yoshihiro; Kawamura, Kazuo; Miyazaki, Hiroshi.

In: FEBS Letters, Vol. 353, No. 1, 10.10.1994, p. 57-61.

Research output: Contribution to journalArticle

Sohma, Y, Akahori, H, Seki, N, Hori, TA, Ogami, K, Kato, T, Shimada, Y, Kawamura, K & Miyazaki, H 1994, 'Molecular cloning and chromosomal localization of the human thrombopoietin gene', FEBS Letters, vol. 353, no. 1, pp. 57-61. https://doi.org/10.1016/0014-5793(94)01008-0
Sohma, Yoshiaki ; Akahori, Hiromichi ; Seki, Naohiko ; Hori, Tada aki ; Ogami, Kinya ; Kato, Takashi ; Shimada, Yoshihiro ; Kawamura, Kazuo ; Miyazaki, Hiroshi. / Molecular cloning and chromosomal localization of the human thrombopoietin gene. In: FEBS Letters. 1994 ; Vol. 353, No. 1. pp. 57-61.
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