Molecular cloning and chromosomal mapping of the mouse gene encoding cyclin-dependent kinase 5 regulatory subunit p35

Toshio Ohshima, Christine A. Kozak, James W. Nagle, Harish C. Pant, Roscoe O. Brady, Ashok B. Kulkarni

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A neural-specific activating subunit, p35, of cyclin-dependent kinase 5 (Cdk5) was recently reported to differ from other mammalian cyclins, suggesting a new type of regulatory subunit for Cdk activity. The mouse gene encoding p35, Cdk5r, was isolated from a mouse 129/SvJ genomic library, and the genomic structure of Cdk5r was characterized. The most notable features of Cdk5r are the absence of introns in the amino acid coding region and the high homology of amino acid sequence among species. The 5'-flanking region of Cdk5r contained no canonical TATA or CAAT box but had several putative promoter elements, including Sp1, AP2, MRE, and NGFIA. The mouse Cdk5r transcript was detected only in the brain by Northern blot analysis. Mouse Cdk5r was mapped to a position on mouse chromosome 11.

Original languageEnglish
Pages (from-to)372-375
Number of pages4
JournalGenomics
Volume35
Issue number2
DOIs
Publication statusPublished - 1996 Jul 15
Externally publishedYes

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Cyclin-Dependent Kinase 5
Chromosome Mapping
Molecular Cloning
129 Strain Mouse
Amino Acid Sequence Homology
Chromosomes, Human, Pair 11
Cyclins
Genomic Library
5' Flanking Region
Northern Blotting
Introns
Amino Acids
Brain
Genes

ASJC Scopus subject areas

  • Genetics

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Molecular cloning and chromosomal mapping of the mouse gene encoding cyclin-dependent kinase 5 regulatory subunit p35. / Ohshima, Toshio; Kozak, Christine A.; Nagle, James W.; Pant, Harish C.; Brady, Roscoe O.; Kulkarni, Ashok B.

In: Genomics, Vol. 35, No. 2, 15.07.1996, p. 372-375.

Research output: Contribution to journalArticle

Ohshima, Toshio ; Kozak, Christine A. ; Nagle, James W. ; Pant, Harish C. ; Brady, Roscoe O. ; Kulkarni, Ashok B. / Molecular cloning and chromosomal mapping of the mouse gene encoding cyclin-dependent kinase 5 regulatory subunit p35. In: Genomics. 1996 ; Vol. 35, No. 2. pp. 372-375.
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