Molecular cloning and expression of long-wavelength-sensitive cone opsin in the brain of a tropical damselfish

Yuki Takeuchi, Mohammad Abu Jafor Bapary, Syugo Igarashi, Satoshi Imamura, Yuji Sawada, Mio Matsumoto, Sung Pyo Hur, Akihiro Takemura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ovarian development of the sapphire devil, Chrysiptera cyanea, exhibits photoperiodism and is stimulated under long-day conditions. Previous studies suggest that red light is more effective than green and blue lights for inducing ovarian development. In addition, the extra-retinal photoreception involved in the ovarian development is suggested in this species. The present study aimed to clone the red-light-sensitive cone opsin (sdLWS) of this species, to demonstrate its expression in the brain, and to confirm the effectiveness of red light on the initiation of ovarian development. A phylogenetic analysis revealed that sdLWS belongs to the long-wavelength-sensitive opsin (LWS opsin) group, showing high identity (77-92%) with the LWS opsin of other fishes. RT-PCR showed that sdLWS is expressed in the eye, brain, and ovary. In situ hybridization indicated that sdLWS is expressed in the third ventricle periventricular area in the anterior hypothalamus. Exposing fish to long-day conditions of red light resulted in the appearance of vitellogenic oocytes in the ovary and an increase in the gonadosomatic index within 2. weeks, suggesting that fish immediately undergo oocyte development under red light conditions. These results indicate that sdLWS is a possible candidate of deep brain photoreceptor molecule involved in photoperiodic ovarian development in the sapphire devil.

Original languageEnglish
Pages (from-to)486-492
Number of pages7
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume160
Issue number4
DOIs
Publication statusPublished - 2011 Dec
Externally publishedYes

Fingerprint

Cone Opsins
Cloning
Molecular Cloning
Brain
Light
Fishes
Fish
Aluminum Oxide
Opsins
Oocytes
Ovary
Methyl Green
Anterior Hypothalamus
Third Ventricle
Wavelength
In Situ Hybridization
Clone Cells
Polymerase Chain Reaction
Molecules

Keywords

  • Coral reef
  • Damselfish
  • Histology
  • Long day
  • Photoreceptor
  • Vitellogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Molecular cloning and expression of long-wavelength-sensitive cone opsin in the brain of a tropical damselfish. / Takeuchi, Yuki; Bapary, Mohammad Abu Jafor; Igarashi, Syugo; Imamura, Satoshi; Sawada, Yuji; Matsumoto, Mio; Hur, Sung Pyo; Takemura, Akihiro.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 160, No. 4, 12.2011, p. 486-492.

Research output: Contribution to journalArticle

Takeuchi, Yuki ; Bapary, Mohammad Abu Jafor ; Igarashi, Syugo ; Imamura, Satoshi ; Sawada, Yuji ; Matsumoto, Mio ; Hur, Sung Pyo ; Takemura, Akihiro. / Molecular cloning and expression of long-wavelength-sensitive cone opsin in the brain of a tropical damselfish. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2011 ; Vol. 160, No. 4. pp. 486-492.
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