Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa

Yuki Oshima, Yoshinobu Uno, Yoichi Matsuda, Tohru Kobayashi, Masahisa Nakamura

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Foxl2 is a transcription factor that plays a crucial role in the regulation of the early development of the female gonad in mammals and fish. However, little is known regarding its role in ovarian differentiation in amphibians. In this study, we isolated a Foxl2 cDNA from the ovary of the frog Rana rugosa and Xenopus laevis and examined its expression during gonadal sex differentiation in R. rugosa. Alignment of known Foxl2 sequences from vertebrates showed high identity of the Foxl2 open reading frame and protein sequences, in particular the forkhead domain and C-terminal region, with other vertebrate sequences. Among different adult tissues, Foxl2 was expressed at its highest level in the ovary. Real-time RT-PCR analysis showed that Foxl2 expression was sexually dimorphic during gonadal sex differentiation in R. rugosa. In addition, Foxl2, which was detected immunochemically in somatic cells surrounding oocytes in the ovary, promoted R. rugosa CYP19 transcription in luciferase promoter assays conducted in A6 cells. We also found by FISH analysis that Foxl2 was an autosomal gene. Altogether, these results suggest that Foxl2 probably plays a very important role in ovarian differentiation of R. rugosa by possibly regulating CYP19 expression. The factor that up-regulates Foxl2 expression in female gonads still remains to be identified.

    Original languageEnglish
    Pages (from-to)170-177
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume159
    Issue number2-3
    DOIs
    Publication statusPublished - 2008 Nov

    Fingerprint

    Ranidae
    Molecular Cloning
    Anura
    frogs
    molecular cloning
    Ovary
    Sex Differentiation
    Aromatase
    Gonads
    Gene Expression
    gene expression
    Vertebrates
    sexual development
    Xenopus laevis
    Amphibians
    gonads
    Luciferases
    Open Reading Frames
    Oocytes
    vertebrates

    Keywords

    • FISH
    • Foxl2
    • Frog
    • Gonad
    • Immunostaining
    • Luciferase assay
    • Sex differentiation

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa. / Oshima, Yuki; Uno, Yoshinobu; Matsuda, Yoichi; Kobayashi, Tohru; Nakamura, Masahisa.

    In: General and Comparative Endocrinology, Vol. 159, No. 2-3, 11.2008, p. 170-177.

    Research output: Contribution to journalArticle

    Oshima, Y, Uno, Y, Matsuda, Y, Kobayashi, T & Nakamura, M 2008, 'Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa', General and Comparative Endocrinology, vol. 159, no. 2-3, pp. 170-177. https://doi.org/10.1016/j.ygcen.2008.08.013
    Oshima, Yuki ; Uno, Yoshinobu ; Matsuda, Yoichi ; Kobayashi, Tohru ; Nakamura, Masahisa. / Molecular cloning and gene expression of Foxl2 in the frog Rana rugosa. In: General and Comparative Endocrinology. 2008 ; Vol. 159, No. 2-3. pp. 170-177.
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