Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo

Masaaki Matsuoka, Yoshiharu Matsuura, Kentaro Senba, Ikuo Nishimoto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

cdk3 has been considered to be rate-limiting for cell cycle progression of mammalian cells while its precise function remains to be elucidated, To assess cdk3 function, a cDNA coding for a cyclin-like protein (designated as ik3-1 from an interactor-1 with cdk3) was isolated with the yeast two-hybrid system using a cyclin-dependent kinase 3 (cdk3) cDNA as bait. p70(ik3-1) (a 70-kDa protein designated as p70(ik3-1)) seems to belong to the cyclin family as its C-terminal domain composed of 124 amino acids resembles the highly conserved cyclin box. Coimmunoprecipitation indicated that p70(ik3-1) binds to p35(cdk3) in vivo. The ik3-1 gene may belong to a multigene family and is highly conserved during evolution. mRNA expression of ik3-1 was low in the early G1 phase, upregulated during G1 progression, maximal at a mid-late G1 point, and declined gradually thereafter, suggesting that it may work mainly in G1 phase. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)442-447
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume273
Issue number2
DOIs
Publication statusPublished - 2000 Jul 5
Externally publishedYes

Fingerprint

Cyclin-Dependent Kinase 3
Cyclins
Cloning
Molecular Cloning
G1 Phase
Proteins
Complementary DNA
Cells
Two-Hybrid System Techniques
Multigene Family
Hybrid systems
Yeast
Cell Cycle
Genes
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo. / Matsuoka, Masaaki; Matsuura, Yoshiharu; Senba, Kentaro; Nishimoto, Ikuo.

In: Biochemical and Biophysical Research Communications, Vol. 273, No. 2, 05.07.2000, p. 442-447.

Research output: Contribution to journalArticle

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