Molecular cloning of bullfrog D2 dopamine receptor cDNA

Tissue distribution of three isoforms of D2 dopamine receptor mRNA

Masaki Nakano, Itaru Hasunuma, Reiko Okada, Kazutoshi Yamamoto, Sakae Kikuyama, Takeo Machida, Tetsuya Kobayashi

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The cDNA encoding D2 dopamine receptor was cloned from the distal lobe of the bullfrog pituitary. The deduced amino acid sequence of the bullfrog D2 dopamine receptor (bfD2A) spanned 444 amino acids and exhibited typical features of those of D2 dopamine receptors cloned in other animals to date. It showed a high similarity of 75-87% with rat, turkey, Xenopus and tilapia counterparts. Further analysis of nucleotide sequence of the cDNA revealed the presence of putative truncated D2 dopamine receptor isoforms, bfD2B and bfD2C, of which nucleotide sequences lacked 12 and 99 nucleotides of the coding region for bfD2A, respectively. The alignment analysis indicated that putative bfD2C isoform was close to D2S subtype cloned in mammals and birds, whereas bfD2A and putative bfD2B isoforms were close to mammalian and avian D2L subtype and homologous to two isoforms of Xenopus. This is the first report of the presence of mRNAs for two D2L-like isoforms and one D2S-like isoform in a single species. The amino acid sequence responsible for producing isoforms is present in the third intracellular loop, which has been shown to play an important role in the coupling with G protein. Accordingly, differences in the mode of coupling with G protein among three isoforms were suggested. The expression of three isoforms mRNA in organs and tissues was analyzed by RT-PCR. In the brain, pars distalis and pars neurointermedia, mRNAs for three isoforms were invariably expressed, whereas only putative bfD2C mRNA was expressed in peripheral organs and tissues.

    Original languageEnglish
    Pages (from-to)143-148
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume168
    Issue number1
    DOIs
    Publication statusPublished - 2010 Aug

    Fingerprint

    Rana catesbeiana
    Dopamine D2 Receptors
    Molecular Cloning
    tissue distribution
    Tissue Distribution
    molecular cloning
    Protein Isoforms
    Complementary DNA
    Messenger RNA
    Xenopus
    G-proteins
    amino acid sequences
    RNA Isoforms
    nucleotide sequences
    tilapia (common name)
    GTP-Binding Proteins
    Amino Acid Sequence
    nucleotides
    reverse transcriptase polymerase chain reaction
    mammals

    Keywords

    • Bullfrog
    • D2 receptor
    • Dopamine
    • Isoform

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Molecular cloning of bullfrog D2 dopamine receptor cDNA : Tissue distribution of three isoforms of D2 dopamine receptor mRNA. / Nakano, Masaki; Hasunuma, Itaru; Okada, Reiko; Yamamoto, Kazutoshi; Kikuyama, Sakae; Machida, Takeo; Kobayashi, Tetsuya.

    In: General and Comparative Endocrinology, Vol. 168, No. 1, 08.2010, p. 143-148.

    Research output: Contribution to journalArticle

    Nakano, Masaki ; Hasunuma, Itaru ; Okada, Reiko ; Yamamoto, Kazutoshi ; Kikuyama, Sakae ; Machida, Takeo ; Kobayashi, Tetsuya. / Molecular cloning of bullfrog D2 dopamine receptor cDNA : Tissue distribution of three isoforms of D2 dopamine receptor mRNA. In: General and Comparative Endocrinology. 2010 ; Vol. 168, No. 1. pp. 143-148.
    @article{7d5acffdacfe4541abe62a12ee54a862,
    title = "Molecular cloning of bullfrog D2 dopamine receptor cDNA: Tissue distribution of three isoforms of D2 dopamine receptor mRNA",
    abstract = "The cDNA encoding D2 dopamine receptor was cloned from the distal lobe of the bullfrog pituitary. The deduced amino acid sequence of the bullfrog D2 dopamine receptor (bfD2A) spanned 444 amino acids and exhibited typical features of those of D2 dopamine receptors cloned in other animals to date. It showed a high similarity of 75-87{\%} with rat, turkey, Xenopus and tilapia counterparts. Further analysis of nucleotide sequence of the cDNA revealed the presence of putative truncated D2 dopamine receptor isoforms, bfD2B and bfD2C, of which nucleotide sequences lacked 12 and 99 nucleotides of the coding region for bfD2A, respectively. The alignment analysis indicated that putative bfD2C isoform was close to D2S subtype cloned in mammals and birds, whereas bfD2A and putative bfD2B isoforms were close to mammalian and avian D2L subtype and homologous to two isoforms of Xenopus. This is the first report of the presence of mRNAs for two D2L-like isoforms and one D2S-like isoform in a single species. The amino acid sequence responsible for producing isoforms is present in the third intracellular loop, which has been shown to play an important role in the coupling with G protein. Accordingly, differences in the mode of coupling with G protein among three isoforms were suggested. The expression of three isoforms mRNA in organs and tissues was analyzed by RT-PCR. In the brain, pars distalis and pars neurointermedia, mRNAs for three isoforms were invariably expressed, whereas only putative bfD2C mRNA was expressed in peripheral organs and tissues.",
    keywords = "Bullfrog, D2 receptor, Dopamine, Isoform",
    author = "Masaki Nakano and Itaru Hasunuma and Reiko Okada and Kazutoshi Yamamoto and Sakae Kikuyama and Takeo Machida and Tetsuya Kobayashi",
    year = "2010",
    month = "8",
    doi = "10.1016/j.ygcen.2010.04.016",
    language = "English",
    volume = "168",
    pages = "143--148",
    journal = "General and Comparative Endocrinology",
    issn = "0016-6480",
    publisher = "Academic Press Inc.",
    number = "1",

    }

    TY - JOUR

    T1 - Molecular cloning of bullfrog D2 dopamine receptor cDNA

    T2 - Tissue distribution of three isoforms of D2 dopamine receptor mRNA

    AU - Nakano, Masaki

    AU - Hasunuma, Itaru

    AU - Okada, Reiko

    AU - Yamamoto, Kazutoshi

    AU - Kikuyama, Sakae

    AU - Machida, Takeo

    AU - Kobayashi, Tetsuya

    PY - 2010/8

    Y1 - 2010/8

    N2 - The cDNA encoding D2 dopamine receptor was cloned from the distal lobe of the bullfrog pituitary. The deduced amino acid sequence of the bullfrog D2 dopamine receptor (bfD2A) spanned 444 amino acids and exhibited typical features of those of D2 dopamine receptors cloned in other animals to date. It showed a high similarity of 75-87% with rat, turkey, Xenopus and tilapia counterparts. Further analysis of nucleotide sequence of the cDNA revealed the presence of putative truncated D2 dopamine receptor isoforms, bfD2B and bfD2C, of which nucleotide sequences lacked 12 and 99 nucleotides of the coding region for bfD2A, respectively. The alignment analysis indicated that putative bfD2C isoform was close to D2S subtype cloned in mammals and birds, whereas bfD2A and putative bfD2B isoforms were close to mammalian and avian D2L subtype and homologous to two isoforms of Xenopus. This is the first report of the presence of mRNAs for two D2L-like isoforms and one D2S-like isoform in a single species. The amino acid sequence responsible for producing isoforms is present in the third intracellular loop, which has been shown to play an important role in the coupling with G protein. Accordingly, differences in the mode of coupling with G protein among three isoforms were suggested. The expression of three isoforms mRNA in organs and tissues was analyzed by RT-PCR. In the brain, pars distalis and pars neurointermedia, mRNAs for three isoforms were invariably expressed, whereas only putative bfD2C mRNA was expressed in peripheral organs and tissues.

    AB - The cDNA encoding D2 dopamine receptor was cloned from the distal lobe of the bullfrog pituitary. The deduced amino acid sequence of the bullfrog D2 dopamine receptor (bfD2A) spanned 444 amino acids and exhibited typical features of those of D2 dopamine receptors cloned in other animals to date. It showed a high similarity of 75-87% with rat, turkey, Xenopus and tilapia counterparts. Further analysis of nucleotide sequence of the cDNA revealed the presence of putative truncated D2 dopamine receptor isoforms, bfD2B and bfD2C, of which nucleotide sequences lacked 12 and 99 nucleotides of the coding region for bfD2A, respectively. The alignment analysis indicated that putative bfD2C isoform was close to D2S subtype cloned in mammals and birds, whereas bfD2A and putative bfD2B isoforms were close to mammalian and avian D2L subtype and homologous to two isoforms of Xenopus. This is the first report of the presence of mRNAs for two D2L-like isoforms and one D2S-like isoform in a single species. The amino acid sequence responsible for producing isoforms is present in the third intracellular loop, which has been shown to play an important role in the coupling with G protein. Accordingly, differences in the mode of coupling with G protein among three isoforms were suggested. The expression of three isoforms mRNA in organs and tissues was analyzed by RT-PCR. In the brain, pars distalis and pars neurointermedia, mRNAs for three isoforms were invariably expressed, whereas only putative bfD2C mRNA was expressed in peripheral organs and tissues.

    KW - Bullfrog

    KW - D2 receptor

    KW - Dopamine

    KW - Isoform

    UR - http://www.scopus.com/inward/record.url?scp=77953699067&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=77953699067&partnerID=8YFLogxK

    U2 - 10.1016/j.ygcen.2010.04.016

    DO - 10.1016/j.ygcen.2010.04.016

    M3 - Article

    VL - 168

    SP - 143

    EP - 148

    JO - General and Comparative Endocrinology

    JF - General and Comparative Endocrinology

    SN - 0016-6480

    IS - 1

    ER -