Abstract
In amphibian larvae, prolactin (PRL) is known to possess growth-promoting and anti-metamorphic activities. For further understanding of the role of PRL in larvae, bullfrog PRL receptor (bfPRLR) cDNA was obtained from the tail fin of premetamorphic tadpoles by use of the reverse transcription-polymerase chain reaction (RT-PCR) coupled with 5′- and 3′- rapid amplification of cDNA ends (RACE). The predicted bfPRLR was composed of 617 amino acids, contained a single transmembrane domain, and showed 33-57% sequence homologies with known sequences of vertebrate PRLRs. When bfPRLR was transiently expressed, specific binding of 125I-labeled bullfrog PRL (bfPRL) was observed. By Northern blot analysis, a 3-kb transcript was detected in the tail fin. By RT-PCR bfPRLR mRNA expression was detected mainly in the brain, kidney, skin, and tail throughout prometamorphic and middle climactic periods. The results of an RNase protection assay revealed that the bfPRLR mRNA level in the tail fin increased around the onset of climax (stage XX) and was maintained at a relatively high value at least until mid-climax (stage XXII). It also revealed that bfPRLR mRNA level in the kidney of larvae gradually rose as metamorphosis progressed. The results support the view that PRL in larval period acts not only on the larval organs but also on the organs that are necessary for the adult life to maintain or develop their structures and functions.
| Original language | English |
|---|---|
| Pages (from-to) | 200-210 |
| Number of pages | 11 |
| Journal | General and Comparative Endocrinology |
| Volume | 138 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 Sep 15 |
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Keywords
- Bullfrog
- Metamorphosis
- Prolactin
- Prolactin receptor
ASJC Scopus subject areas
- Endocrinology
Cite this
Molecular cloning of bullfrog prolactin receptor cDNA : Changes in prolactin receptor mRNA level during metamorphosis. / Hasunuma, Itaru; Yamamoto, Kazutoshi; Kikuyama, Sakae.
In: General and Comparative Endocrinology, Vol. 138, No. 3, 15.09.2004, p. 200-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of bullfrog prolactin receptor cDNA
T2 - Changes in prolactin receptor mRNA level during metamorphosis
AU - Hasunuma, Itaru
AU - Yamamoto, Kazutoshi
AU - Kikuyama, Sakae
PY - 2004/9/15
Y1 - 2004/9/15
N2 - In amphibian larvae, prolactin (PRL) is known to possess growth-promoting and anti-metamorphic activities. For further understanding of the role of PRL in larvae, bullfrog PRL receptor (bfPRLR) cDNA was obtained from the tail fin of premetamorphic tadpoles by use of the reverse transcription-polymerase chain reaction (RT-PCR) coupled with 5′- and 3′- rapid amplification of cDNA ends (RACE). The predicted bfPRLR was composed of 617 amino acids, contained a single transmembrane domain, and showed 33-57% sequence homologies with known sequences of vertebrate PRLRs. When bfPRLR was transiently expressed, specific binding of 125I-labeled bullfrog PRL (bfPRL) was observed. By Northern blot analysis, a 3-kb transcript was detected in the tail fin. By RT-PCR bfPRLR mRNA expression was detected mainly in the brain, kidney, skin, and tail throughout prometamorphic and middle climactic periods. The results of an RNase protection assay revealed that the bfPRLR mRNA level in the tail fin increased around the onset of climax (stage XX) and was maintained at a relatively high value at least until mid-climax (stage XXII). It also revealed that bfPRLR mRNA level in the kidney of larvae gradually rose as metamorphosis progressed. The results support the view that PRL in larval period acts not only on the larval organs but also on the organs that are necessary for the adult life to maintain or develop their structures and functions.
AB - In amphibian larvae, prolactin (PRL) is known to possess growth-promoting and anti-metamorphic activities. For further understanding of the role of PRL in larvae, bullfrog PRL receptor (bfPRLR) cDNA was obtained from the tail fin of premetamorphic tadpoles by use of the reverse transcription-polymerase chain reaction (RT-PCR) coupled with 5′- and 3′- rapid amplification of cDNA ends (RACE). The predicted bfPRLR was composed of 617 amino acids, contained a single transmembrane domain, and showed 33-57% sequence homologies with known sequences of vertebrate PRLRs. When bfPRLR was transiently expressed, specific binding of 125I-labeled bullfrog PRL (bfPRL) was observed. By Northern blot analysis, a 3-kb transcript was detected in the tail fin. By RT-PCR bfPRLR mRNA expression was detected mainly in the brain, kidney, skin, and tail throughout prometamorphic and middle climactic periods. The results of an RNase protection assay revealed that the bfPRLR mRNA level in the tail fin increased around the onset of climax (stage XX) and was maintained at a relatively high value at least until mid-climax (stage XXII). It also revealed that bfPRLR mRNA level in the kidney of larvae gradually rose as metamorphosis progressed. The results support the view that PRL in larval period acts not only on the larval organs but also on the organs that are necessary for the adult life to maintain or develop their structures and functions.
KW - Bullfrog
KW - Metamorphosis
KW - Prolactin
KW - Prolactin receptor
UR - http://www.scopus.com/inward/record.url?scp=4444220233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4444220233&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2004.06.005
DO - 10.1016/j.ygcen.2004.06.005
M3 - Article
VL - 138
SP - 200
EP - 210
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
SN - 0016-6480
IS - 3
ER -