Molecular cloning of Dmrt1 and its expression in the gonad of Xenopus

Noboru Osawa, Yuki Oshima, Masahisa Nakamura

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    Sex is determined by various transcription factors in vertebrates. SRY on the Y chromosome in mammals has been isolated as the sex-determining gene. In birds, Dmrt1 on the Z chromosome of chickens is expressed in the indifferent gonad before sex determination. In amphibians, the sex-determining gene is not known yet. To examine whether Dmrt1 is expressed during sex determination in Xenopus laevis, we first isolated the cDNA, and examined its expression in gonadal development by RT-PCR analysis. The expression of Xenopus Dmrt1 (xDmrt1) was observed exclusively in the testis amosis. The expression was much higher in the testis than in the ovary. This was a pattern different from that in human, mouse, and fish such as tilapia and rainbow trout. In addition, the Dmrt1 mRNA was first detected in the gonad/mesonephros complex of tadpoles at stage 53, at which time the gonads were still indifferent. Taken together, the results suggest that Dmrt1 may be involved in gonadal differentiation of Xenopus.

    Original languageEnglish
    Pages (from-to)681-687
    Number of pages7
    JournalZoological Science
    Volume22
    Issue number6
    DOIs
    Publication statusPublished - 2005 Jun

    Fingerprint

    Xenopus
    gonads
    molecular cloning
    gender
    testes
    Z chromosome
    Xenopus laevis
    Y chromosome
    tilapia (common name)
    tadpoles
    amphibians
    Oncorhynchus mykiss
    genes
    transcription factors
    reverse transcriptase polymerase chain reaction
    vertebrates
    mammals
    chickens
    birds
    mice

    Keywords

    • cDNA
    • Dmrt1
    • Gonadal differentiation
    • RT-PCR
    • Sox9
    • X. laevis

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Molecular cloning of Dmrt1 and its expression in the gonad of Xenopus. / Osawa, Noboru; Oshima, Yuki; Nakamura, Masahisa.

    In: Zoological Science, Vol. 22, No. 6, 06.2005, p. 681-687.

    Research output: Contribution to journalArticle

    Osawa, Noboru ; Oshima, Yuki ; Nakamura, Masahisa. / Molecular cloning of Dmrt1 and its expression in the gonad of Xenopus. In: Zoological Science. 2005 ; Vol. 22, No. 6. pp. 681-687.
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